- Regulatory Status
- RUO
- Other Names
- CD86, B7-2, T lymphocyte activation antigen CD86, Activation B7-2 antigen, CD28 antigen ligand 2, CD28LG2B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
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Immobilized recombinant human B7-2/CD86-Fc binds biotinylated human CTLA-4 in a dose dependent manner with EC50 of 10 - 40 ng/mL. -
Stability testing for Human B7-2/CD86-Fc. Human B7-2/CD86-Fc was aliquoted in PBS, pH 7.4 at 0.2 mg/mL. One aliquot was freeze-thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested for their ability to bind biotinylated human CTLA-4.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 775602 | 10 µg | $71.00 | |||
| 775604 | 25 µg | $106.00 | |||
| 775606 | 100 µg | $223.00 | |||
| 775608 | 500 µg | $645.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD86, also known as B7-2, is a member of the cell surface immunoglobulin superfamily. B7 family members are transmembrane molecules that exist predominantly as monomers. They are classified into three groups according to their functions. Group I members include B7-1, B7-2, and B7-H2. These members are either co-stimulatory or co-inhibitory in modulating T cell response. Group II members include B7-H1 (PD-L1) and B7-DC (PD-L2) which are ligands for PD-1. These members are involved in regulating induction and maintenance of peripheral immune tolerance. Group III members include B7-H3 and B7-H4 whose role in T cell regulation has yet to be defined. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Product Details
- Source
- Human B7-2/CD86, amino acid Leu20-His245 (accession # P42081) with a C-terminal human IgG1 (Pro100-Lys330) and 6x His tag expressed in 293E cells.
- Molecular Mass
- The 465 amino acid recombinant protein has a predicted molecular mass of approximately 52.9 kD. The DTT-reduced protein migrates at approximately 70 kD and non-reduced protein migrates at approximately 140 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 1x PBS.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When human B7-2/CD86-Fc is immobilized at 0.5 µg/mL (100 µL/ well), biotinylated human CTLA-4 binds with EC50 of 10 - 40 ng/mL in a functional ELISA.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Immunoglobulin family
- Distribution
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Activated antigen presenting cells, including B cells, dendritic cells and macrophages
- Function
- B7-2 is a costimulatory molecule that is required for T cell proliferation and IL-2 production.
- Interaction
- Leukocytes, endothelial cells
- Ligand/Receptor
- CD28, CTLA4
- Bioactivity
- Measured by the ability of immobilized protein to bind biotinylated human CTLA-4
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
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- Freeman GJ, et al. 1993. Science 262:909.
- Chen C, et al. 1994. J Immunol. 152:4929.
- Lanier LL, et al. 1995. J Immunol. 154:97.
- Carreno BM, et al. 2002. Annu Rev Immunol. 20:29.
- Rudd CE, et al. 2009. Immunol Rev. 229:12.
- Wing K, et al. 2011. Trends Immunol. 32:428.
- Gene ID
- 942 View all products for this Gene ID
- UniProt
- View information about B7-2/CD86 on UniProt.org