- Regulatory Status
- RUO
- Other Names
- Bone morphogenetic protein 4 (BMP4), Bone morphogenetic protein 2B (BMP-2B)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 710002 | 10 µg | $253.00 | |||
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BMP-4 is a member of the bone morphogenetic protein (BMP) family of cytokines, which belong to the transforming growth factor beta (TGF-β) superfamily. BMP-4 plays a crucial function in the chondrogenic and osteogenic differentiation of mesenchymal stem cells as well as in the maintenance of adult cartilage and bone. Binding of BMP-4 to the BMPR-I/BMPR-II receptor complex was reported to activate several signaling pathways, such as the Smad, the MAPK, the PI3K/Akt, and the Wnt pathways, which are involved in bone formation, cell proliferation, and cell differentiation. Genetic defects in BMP-4 have been associated with microphthalmia and orofacial cleft.
Product Details
- Source
- Human BMP-4, amino acids (Lys303-Arg408) (Accession# NP_001193.1), was expressed in E. coli.
- Molecular Mass
- The 106 amino acid recombinant protein has a predicted molecular mass of approximately 11.9 kD. Recombinant BMP-4 is disulphide bond-linked homodimer. The predicted N-terminal amino acid is Lys.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE gel and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 5 - 10 ng/ml, corresponding to a specific activity of 1 - 2 x 105 units/mg, as measured by its ability to induce alkaline phosphatase production by ATDC-5 cells in a dose dependent manner.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human, mouse, and rat.
- Product Citations
-
Antigen Details
- Structure
- Growth factor
- Distribution
- BMP-4 is widely expressed.
- Function
- BMP-4 is a multifunctional growth factor and is involved in bone formation, tooth development, and fracture repair.
- Interaction
- BMP-4 forms homodiner and interacts with GREM2, HTRA1, HTRA3, SOSTDC1.
- Ligand/Receptor
- BMP-4 binds to the receptor BMPR-I and BMPR-II.
- Bioactivity
- Human BMP-4 is able to induce alkaline phosphatase production in ATDC-5 cells.
- Cell Type
- Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Kishimoto KN, et al. 2013. Biomed. Res. Int. 2013:143742.
2. Kuroda K, et al. 2013. Skelet. Muscle. 3:5.
3. Wei X, et al. 2013. Cell. Prolif. 46:416.
4. Yacqub-Usman K, et al. 2012. Endocrinology. 153:3603.
5. Tan TW, et al. 2012. J. Cell Physiol. 227:2531.
6. Labeur M, et al. 2010. Mol. Cell. Endocrinol. 326:85. - Gene ID
- 652 View all products for this Gene ID
- UniProt
- View information about BMP-4 on UniProt.org