- Regulatory Status
- RUO
- Other Names
- Bone morphogenetic protein 4, BMP-2B, DVR4
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 595201 | 2 µg | $94.00 | |||
| 595202 | 10 µg | $218.00 | |||
This product is not available for shipping outside of the United States.
Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGFbeta) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II / IIB activin receptors. BMP-4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, and zebrafish. BMP-2 and BMP-4 are highly similar; they have 92% amino acid sequence identity. BMP-4 is required for mesoderm formation and patterning in mice, and BMP-4 KO mice are embryonic-lethal. BMP-4 has an important role in postnatal tooth cytodifferentiation. Overexpression BMP-4 has been associated with fibrodysplasia ossificans progressiva (FOP). Overexpression of BMP-4 has also been associated with cancer, including melanoma, hepatocellular carcinoma, adenocarcinoma and colorectal cancer cell lines. This overexpression can promote migratory and invasive properties of cancer cells.
Product Details
- Source
- Human BMP-4, amino acids (Lys303 -Arg408) (Accession# NM_001202), was expressed in E. coli.
- Molecular Mass
- The 106 amino acid recombinant protein has a predicted molecular mass of approximately 12 kD. The DTT-non reduced protein migrates at approximately 31-36 kD on SDS-PAGE. The predicted N-terminal amino acid is Lys.
- Purity
- >97%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- BMP-4 induces alkaline phosphatase production on ATDC-5 cells. The expected ED50 for this effect is 5-10 ng/ml, corresponding to 1-2 x 105 unit/mg.
- Application
-
Bioassay
- Product Citations
-
Antigen Details
- Structure
- Growth factor, homodimer
- Distribution
- Osteoblast, odontoblast, ameloblasts, megakaryocytes, endothelial cells. Lymphoblastoid cell lines from patients with fibrodysplasia ossificans progressiva.
- Function
- BMP-4 induces endochondral osteogenesis, fracture healing, and tooth development.
- Interaction
- Condroblast, osteoblast, retinoblastoma cells.
- Ligand/Receptor
- Type I receptors BMPR-IA (ALK-3) and BMPR-IB (ALK-6).
- Cell Type
- Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. ten Dijke, et al. 1994. J. Biol. Chem. 269:16985.
2. Shafritz AB, et al. 1996. New Eng. J. Med. 335:555.
3. Chen D, et al. 2004. Growth Factors 22:233.
4. Gluhak-Heinrich J, et al. 2010. Bone 46:1533.
5. Haubold M, et al. 2010. Int. J. Biol. Sci. 6:700. - Gene ID
- 652 View all products for this Gene ID
- UniProt
- View information about BMP-4 on UniProt.org