- Regulatory Status
- RUO
- Other Names
- Bone morphogenetic protein 5
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 595402 | 10 µg | $218.00 | |||
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Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. Nervous system development involves several BMPs including BMP-2, 4, 5, and 7. BMP-5 is widely expressed in the nervous system throughout development and into adulthood, and BMP-5 induces multiple dendritic processes to sympathetic neurons in culture. BMP-5 plays an important role in fracture healing during the stage of cartilage formation and periosteal response. In addition, BMP-4 and BMP-5 are expressed in normal synovial tissue and have been found to be decreased in osteoarthritis and rheumatoid arthritis; therefore, it has been speculated that these BMPs are important in joint homeostasis.
Product Details
- Source
- Human BMP-5, amino acids (Ala317-His454) (Accession# NM_021073), was expressed in CHO cells.
- Molecular Mass
- The 138 amino acid recombinant protein has a predicted molecular mass of approximately 15.6 kD. Recombinant human BMP-5 is a homodimeric disulfide-linked protein. The predicted N-terminal amino acid is Ala.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- BMP-5 induces alkaline phosphatase production on ATDC-5 cells. The expected ED50 = 0.5 - 1.0 µg/ml, corresponding to a specific activity of 1.0 - 2.0 x 103 unit/mg.
- Application
-
Bioassay
Antigen Details
- Structure
- Growth factor, homodimer
- Distribution
-
Chondrocytes, neurons.
- Function
- BMP-5 induces cartilaginous differentiation, chondrocyte cell proliferation, and cartilage matrix synthesis. It also regulates dendritic growth in sympathetic neurons. Noggin and follistatin inhibit the dendrite-promoting activity of BMP-5.
- Interaction
- Chondroblasts, osteoblasts, neurons.
- Ligand/Receptor
- Type I receptors BMPR-IA (ALK-3) and type II receptors.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Beck HN, et al. 2001. BMC Neurosci. 2:12.
2. Chen D, et al. 2004. Growth Factors 22:233.
3. Mailhot G, et al. 2008. J. Cell Physiol. 214:56.
4. Ai-Aql ZS, et al. 2008. J. Dent. Res. 87:107.
5. Gluhak-Heinrich J, et al. 2010. Bone 46:1533. - Gene ID
- 653 View all products for this Gene ID
- UniProt
- View information about BMP-5 on UniProt.org