- Regulatory Status
- RUO
- Other Names
- Bone Morphogenetic Protein 6, BMP6, VGR1, VGR, VGR-1
-
Recombinant human BMP-6 induces the production of alkaline phosphatase in ATDC-5 mouse chondrogenic cell line in a dose-dependent manner. The ED50 for this effect is 9 – 56 ng/mL. -
Stability Testing for Recombinant Human BMP-6. Recombinant human BMP-6 was aliquoted in 0.1% Trifluoroacetic acid (TFA) at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to induce the production of alkaline phosphatase in ATDC-5 mouse chondrogenic cell line in a dose-dependent manner. The ED50 for this effect is 9 – 56 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 569304 | 25 µg | $575.00 | |||
| 569306 | 100 µg | $1095.00 | |||
Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four Type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I (ALK-1). Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. Human BMP-6 can restore bone in animal models of osteoporosis and induce bone formation through IGF-I and EGF pathways in a murine model of osteopenia and in human osteoblasts. Human BMP-6 has been reported to have an antiproliferative effect on human B, human CD4 T, Jurkat cells and mouse peritoneal macrophages. In addition, BMP-6 induces expression of pro-inflammatory inducible nitric oxide synthase (iNOS), TNF-α and IL-1β in macrophages. BMP-6 is a key endogenous regulator of hepcidin expression and iron metabolism in vivo. In fact, BMP-6 null mice have a phenotype that resembles mouse models of juvenile hemochromatosis (an iron overload disorder caused by mutations in the genes encoding the major iron regulatory hormone hepcidin and hemojuvelin).
Product Details
- Source
- Recombinant Human BMP-6, amino acid sequence (Gln382-His513) (Accession: # P22004), was expressed in CHO cells. The amino-terminus contains 6His-GGS tag.
- Molecular Mass
- The 132 amino acid recombinant protein has a predicted molecular mass of approximately 14.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 18 kD and 40 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is His.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 0.1% Trifluoroacetic acid (TFA)
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human BMP-6 induces the production of alkaline phosphatase in ATDC-5 mouse chondrogenic cell line in a dose-dependent manner. The ED50 for this effect is 9 – 56 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
-
Oocytes, granulosa cells, bone marrow-mesenchymal stem cells, hematopoietic stem cells, embryonic stem cells, mesenchymal stem cells, neural stem cells, corneal epithelial, and osteoclasts
- Function
- Osteoblast differentiation, bone regeneration. Controls the self-renewal and differentiation of stem cells. Antiproliferative effect on B, T cells, and macrophages. Regulates hepcidin expression and iron metabolism in vivo.
- Interaction
- Cancer cells, mesenchymal stem cells, germline stem cells, hematopoietic stem cells, and neural stem cells
- Ligand/Receptor
- BMP receptors Alk-2 and Alk-3 (type I), BMPRII (type II)
- Bioactivity
- BMP-6 induces alkaline phosphatase in ATDC-5 mouse chondrogenic cell line.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Chen D, et al. 2004. Growth Factors. 22:233-41.
- Kertein C, et al. 2005. BMC Immunol. 6:9.
- Grasser WA, et al. 2007. Int Orthop. 31:759-65.
- Sivertsen EA, et al. 2007. Eur J Immunol. 37:2937-48.
- Andriopoulos B, et al. 2009. Nat Genetics. 41:482-7.
- Hong JH, et al. 2009. Immunology. 128:e442-50.
- Lee GT, et al. 2011. Mol Immunol. 48:1540-7.
- Shen R, et al. 2015. Curr Pharm Des. 21:3634-43.
- Tiwari A, et al. 2020. Invest Ophthalmol Vis Sci. 61:46.
- Gene ID
- 654 View all products for this Gene ID
- UniProt
- View information about BMP-6 on UniProt.org