- Regulatory Status
- RUO
- Other Names
- Bone morphogenetic protein 7, Osteogenic protein 1 (OP-1), Eptotermin alpha
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 595601 | 2 µg | $94.00 | |||
| 595602 | 10 µg | $218.00 | |||
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Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, and are composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. BMP-7, originally identified by its properties to induce bone formation, has been identified as an anti-fibrotic molecule, antagonizing TGF-β1. Fibrosis is associated with the emergence of fibroblasts derived from endothelial cells, suggesting an endothelial-mesenchymal transition (EndMT). TGF-β1 induces endothelial cells to undergo EndMT, whereas bone BMP-7 preserved the endothelial phenotype. BMP-7 inhibits fibrosis in the kidney, lung, liver, heart, peritonium, oral submucosa tissue, and colonic wall.
Product Details
- Source
- Human BMP-7, amino acids (Met315-His431) (Accession# NM_001719), was expressed in CHO cells.
- Molecular Mass
- The 117 amino acid recombinant protein has a predicted molecular mass of approximately 13 kD. Recombinant human BMP-7 is a 28.8 kD homodimeric glycoprotein. The predicted N-terminal amino acid is Met.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- BMP-7 induces alkaline phosphatase production on ATDC-5 cells. The ED50 = 0.02 - 0.04 µg/ml, corresponding to a specific activity of 2.5 - 5.0 x 104 unit/mg.
- Application
-
Bioassay
Antigen Details
- Structure
- Growth factor, homodimer
- Distribution
- Widely expressed.
- Function
- BMP-7 promotes bone formation; BMP-7 induces Langerhans cell differentiation and proliferation, induces brown adipocyte differentiation and thermogenesis, and it has antifibrotic properties.
- Interaction
- Osteoblasts, Langerhans cells, adipocytes, endothelial cells.
- Ligand/Receptor
- BMP type-I receptor ALK3 and Type II BMP receptor.
- Cell Type
- Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Chen D, et al. 2004. Growth Factors 22:233.
2. Tseng Y, et al. 2008. Nature 454:1000.
3. Zeisberg E, et al. 2007. Nat. Med. 13:952.
4. Hao ZM, et al. 2012. Mol. Ther. 20:2043.
5. Yasmin N, et al. 2013. J. Exp. Med. 210:2597. - Gene ID
- 655 View all products for this Gene ID
- UniProt
- View information about BMP-7 on UniProt.org