- Regulatory Status
- RUO
- Other Names
- CD4, CD4 antigen (p55), CD4 antigen, CD4 molecule, CD4 receptor,T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
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Immobilized human CD4 at 2 µg/mL binds to recombinant human biotinylated HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein in a dose-dependent manner. The ED50 for this effect is 27 – 162 ng/mL. -
Stability Testing for Recombinant Human CD4. Recombinant human CD4 was aliquoted in PBS, 2mM CHAPS, and pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were evaluated in a binding assay with immobilized human CD4 at 2 µg/mL and different concentration of recombinant human biotinylated HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein that binds in a dose-dependent manner. The ED50 for this effect is 27 – 162 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 569604 | 25 µg | $205.00 | |||
| 569606 | 100 µg | $415.00 | |||
Cluster of differentiation 4 (CD4), also known as T4 and L3T4, is a member of the immunoglobulin superfamily and single-chain type I transmembrane glycoprotein that plays a crucial role in the immune system including T-cell activation, HIV infection, and other immune-related processes. CD4 is a commonly used marker for the identification of T cell populations. CD4 is found on the surface of immune cells such as monocytes, thymocytes, neutrophils, a subset of mature T lymphocytes, NK cells, macrophages, dendritic cells and neurons. CD4+ T helper cells are an essential part of the human immune system and send signals to other types of immune cells including CD8 killer cells. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. CD4 also acts as a co-receptor for the T-cell receptor (TCR) and is involved in the recognition of antigens presented by antigen-presenting cells. CD4 binds directly to MHC class II molecules on the surface of antigen presenting cells.
Product Details
- Source
- Recombinant human CD4, amino acid Lys26-Trp390 (Accession No. P01730) was expressed in CHO cells. The C-terminal includes 6His.
- Molecular Mass
- The 396 amino acid recombinant protein has a predicted molecular mass of approximately 44.23 kD. The DTT-reduced and non-reduced proteins migrates at approximately 40-55 kD by SDS-PAGE. The N-terminal amino acid is Lys.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, 2 mM CHAPS, and pH 7.2
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized human CD4 at 2 µg/mL binds to recombinant human biotinylated HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein in a dose-dependent manner. The ED50 for this effect is 27 – 162 ng/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Majority of thymocytes, T cell subset, macrophages, neutrophils, monocytes, NK cells, and neurons
- Function
- TCR co-receptor, T cell activation
- Interaction
- Dendritic cells, T cells, Thymocytes, Tregs
- Ligand/Receptor
- MHC class II molecule
- Bioactivity
- Measured by its ability to bind human biotinylated HIV-1 [HIV-1/Clade B/C (CN54)] GP120 protein
- Cell Type
- Monocytes, NKT cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
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- Maddon PJ, et al. 1985. Cell. 42:93-104.
- Kitchen SG, et al. 2005. Proc Natl Acad Sci. 102:3794-9.
- Huppa JB, et al. 2010. Nature. 463:963.
- Agrez M, et al. 2023. Sci Rep. 13:19741.
- Klatzmann D, et al. 1984. Nature. 312:767-8.
- Kim PW, et al. 2023. Science. 301:1725.
- Bofill M, et al. 1992. Clinical and Experimental Immunology. 88:243-52.
- Gene ID
- 920 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org