- Regulatory Status
- RUO
- Other Names
- Carcinoembryonic antigen-related cell adhesion molecule 8, CEACAM-8, CEACAM8, CD67 antigen, CD66b, Carcinoembryonic antigen CGM6, Nonspecific cross-reacting antigen NCA-95
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When Recombinant Human Galectin-3 is immobilized at 2 µg/mL, Recombinant Human CEACAM-8/CD66b binds in a dose dependent manner with EC50 of 50 – 200 ng/mL. -
Stability testing for Human CEACAM-8/CD66b. Human CEACAM-8/CD66b was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaws), and compared to a control kept at 4°C (Control). The samples were tested for their ability to bind Recombinant Human Galectin-3.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 778102 | 10 µg | $106.00 | |||
| 778104 | 25 µg | $206.00 | |||
| 778106 | 100 µg | $616.00 | |||
| 778108 | 500 µg | $1406.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CEACAM-8, also known as CD66b and CD67, is a GPI-linked protein belonging to the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. A homolog of CEACAM-8 has not been identified in rodents. CEACAM-8 is exclusively expressed on human granulocytes, including neutrophils and eosinophils, and is characterized as a granulocyte activation marker. CEACAM-8 is mainly found in secondary granules with low amounts on cell the surface. Upon activation of granulocytes with various stimuli, CEACAM-8 is rapidly upregulated and released from intracellular granules, leading to a high level of expression on the plasma membrane. CEACAM-8 has been reported to be involved in immune response, leukocyte migration, cell adhesion and degranulation. Engagement of CEACAM-8 with Galectin-3 activates a Src kinase, Hck, inducing cell adhesion and degranulation. In addition, CEACAM-8 constitutively associates integrin β2 and CD11b in lipid rafts, regulating cell adhesion and activation of eosinophils. CEACAM-8 can specifically bind CEACAM-6 (CD66c) through their Ig-like V-type domain in calcium-independent manner. The interaction of CEACAM-8 and CEACAM-6 mediates heterophilic cell adhesion.
Product Details
- Source
- Human CEACAM-8, amino acid (Gln35-Asp320) (Accession: #P31997.2) with a C-terminal 6x His tag, was expressed in 293E cells.
- Molecular Mass
- The 292 amino acid recombinant protein has a predicted molecular mass of approximately 32.3 kD. The DTT-reduced and non-reduced protein migrates at approximately 60 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When human Galectin-3 (Cat. No. 599704) is immobilized at 2 µg/mL, human CEACAM-8/CD66b binds with EC50 of 50 - 200 ng/mL in a functional ELISA.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- IgG superfamily
- Distribution
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Restricted to bone marrow, granulocytes; secondary granules, plasma membrane, GPI-anchored
- Function
- Cell adhesion, activation of neutrophils and eosinophils
- Interaction
- CEACAM-6 (CD66c), CD11b
- Ligand/Receptor
- Galectin-3
- Bioactivity
- Measured by Galectin-3 binding assay
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Kuijpers T, et al. 1993. J Immunol. 151: 4934.
- Kuroki M, et al. 1992. J Leuk Biol. 52: 551.
- Ducker TP, et al. 1992. J Leuk Biol. 52: 11.
- Yoon J, et al. 2007. J Immunol. 179: 8454.
- Oikawa S, et al. 2000. Biochem Biophys Res Commun. 278: 564.
- Zhao L, et al. 2002. J Immunol Methods. 270: 27.
- Yamanka T, et al. 1996. Biochem Biophys Res Commun. 219: 842.
- Oikawa S, et al. 1991. J Biol Chem. 266: 7995.
- Gene ID
- 1088 View all products for this Gene ID
- UniProt
- View information about CEACAM-8/CD66b on UniProt.org