- Regulatory Status
- RUO
- Other Names
- Ciliary neurotrophic factor (CNTF)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 710104 | 20 µg | $253.00 | |||
This product is not available for shipping outside of the United States.
CNTF is a member of the four-helix bundle cytokine family and is structurally related to other family members such as IL-6, IL-11, and LIF. Binding of CNTF to its receptor CNTFRα triggers the association with the IL-6 receptor gp130 and the LIF receptor LIFRβ, followed by activating the JAK-STAT, the ERK, and the PI3K signaling pathways. CNTF was first identified as a neuroprotective factor that supported the survival of embryonic chick ciliary neurons. CNTF has been shown to regulate the differentiation and survival of a variety of neuronal cells and has been applied as the treatment for retinal degenerative diseases caused by genetic defect or damage induced by toxins, auto-antibodies, or strong light. CNTF is also revealed as a myotrophic factor for denervated skeletal muscle and a modulator for AMPK-mediated energy balance control.
Product Details
- Source
- Human CNTF, amino acids (Ala2 - Met200) (Accession# NP_000032.1), was expressed in E. coli.
- Molecular Mass
- The 199 amino acid recombinant protein has a predicted molecular mass of approximately 22.8 kD. The predicted N-terminal amino acid is Ala.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in 5-10 mM sodium phosphate, pH 7.5 to a concentration of 0.1-1.0 mg/ml. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 50 - 150 ng/ml, corresponding to a specific activity of 0.67 - 2.0 x 104 units/mg, as measured by its ability to stimulate proliferation of human TF-1 cells in a dose dependent manner.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human, mouse, rat, bacteria, frog, and hamster.
Antigen Details
- Structure
- Growth factor
- Distribution
- CNTF is mainly expressed in glial cells.
- Function
- CNTF has neuroprotective function, regulating survival and differentiation of neurons in the nervous system. CNTF can also act as a myotrophic factor.
- Interaction
- CNTF interacts with skeletal muscle and a variety of neurons in the nervous system.
- Ligand/Receptor
- CNTF binds to a receptor complex that consists of CNTF receptor alpha (CNTFRα), gp130, and LIF receptor beta (LIFRβ).
- Bioactivity
- Human CNTF is able to stimulate proliferation of TF-1 cells.
- Cell Type
- Neural Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Wen R, et al. 2012. Prog. Retin. Eye Res. 31:136.
2. Purser MJ, et al. 2013. PLoS One. 8:e61616.
3. Rhee KD, et al. 2013. Proc Natl Acad Sci USA. 110:E4520.
4. Garcia N, et al. 2012. J. Peripher. Nerv. Syst. 17:312.
5. Vergara C, et al. 2004. Brain Res. Brain Res. Rev. 47:161.
6. Ji JZ, et al. 2004. Eur. J. Neurosci. 19:265. - Gene ID
- 1270 View all products for this Gene ID
- UniProt
- View information about CNTF on UniProt.org