- Regulatory Status
- RUO
- Other Names
- Dickkopf-related protein 1, DKK1, Dickkopf WNT signaling pathway inhibitor 1
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Recombinant Human DKK-1 inhibits TCF reporter activity induced by 50 ng/mL of mouse Wnt3a (Cat. No. 772301) in a dose dependent manner. ED50 for this effect is 6 – 30 ng/mL. -
Stability testing for Human DKK-1. Human DKK-1 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaws), and compared to a control kept at 4°C (Control). The samples were tested for their ability to inhibit TCF reporter activity induced by 50 ng/mL of mouse Wnt3a (Cat. No. 772301).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 778602 | 10 µg | $218.00 | |||
| 778604 | 25 µg | $364.00 | |||
| 778606 | 100 µg | $1113.00 | |||
DKK-1 (Dickkopf related protein 1) is a member of the Dickkopf family of proteins that include DKK-1, -2, -3,-4, and Soggy. DKK-1 was discovered by its ability to block Wnt signaling during early Xenopus embryogenesis. DKK-1 antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt proteins. DKK-1 forms a ternary complex with LRP5/6 and the transmembane protein Kremen1/2, promoting internalization of LRP5/6 and down regulation of Wnt signaling. DKK-1 contains two conserved cysteine-rich domains separated by a linker region. The N-terminal cysteine-rich domain is unique to DKKs while the C-terminal cysteine-rich domain has a pattern of 10 cysteines similar to colipase. DKK-1 binds with LRP6 with high affinity and this interaction is enhanced by Kremens. The interactions between DKK-1 and both LRP6 and Kremens are mediated via C-terminal cysteine-rich domain. DKKs are expressed throughout development and play an important role by locally inhibiting Wnt regulated processes such as limb development, somitogenesis, antero-posterior axial patterning, and eye formation. In the adult, DKKs are implicated in bone formation and bone disease, cancer and Alzheimer's disease. The balance between Wnt signaling and DKK-1 inhibition is important for bone homeostasis. Increased expression of DKK-1 are found in lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers. DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
Product Details
- Source
- Human DKK-1, amino acid Thr32-His266 (Accession #O94907.1) was expressed in 293E cells.
- Molecular Mass
- The 235 amino acid recombinant protein has a predicted molecular mass of approximately 25.78 kD. The DTT-reduced and non-reduced protein migrates at approximately 36-45 kD by SDS-PAGE. The predicted N-terminal amino acid is Thr.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Measured by its ability to inhibit mWnt3a (Cat. No. 772301) induced TCF reporter activity in HEK293T human embryonic kidney cells. ED50 = 6 – 30 ng/mL.
- Application
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Bioassay
- Application Notes
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- BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Distribution
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Placenta, prostate, colon, spleen, and platelets
- Function
- Wnt antagonist implicated in bone formation and bone disease, cancer and Alzheimer's disease. Also promotes type 2 cell-mediated inflammation.
- Interaction
- Bone
- Ligand/Receptor
- LRP5/6, Kremen-1, and Kremen-2.
- Bioactivity
- Inhibits mWnt3a activity in TCF reporter assay
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Stem Cells, Cancer Biomarkers, Neuroscience, Neuroinflammation
- Antigen References
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- Fedi P, et al. 1999. J Biol Chem. 274:19465-72.
- Krupnik VE, et al. 1999. Gene. 238:301-13.
- Mao B, et al. 2001. Nature. 411:321-325.
- Mukhopadhyay M, et al. 2001. Dev Cell. 1:423-434.
- Tian E, et al. 2003. New Eng J Med. 349:2483-2494.
- Niehrs C. 2006. Oncogene. 25:7469-7481.
- Diarra D, et al. 2007. Nature Med. 12:156-163.
- Fujita K and Janz S. 2007. Molecular Cancer. 6:71.
- Ahn VE, et al. 2011. Dev Cell. 21:862-873.
- Gene ID
- 22943 View all products for this Gene ID
- UniProt
- View information about DKK-1 on UniProt.org