- Regulatory Status
- RUO
- Other Names
- SELE, CD62 antigen-like family member E (CD62E), Endothelial leukocyte adhesion molecule 1 (ELAM-1), Leukocyte-endothelial cell adhesion molecule 2 (LECAM2)
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Immobilized recombinant human E-Selectin induces adhesion of U937 cells in a dose dependent manner. ED50 for this effect is 15 - 60 ng/mL. Calcein-AM was used to measure the number of adherent cells.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 718702 | 10 µg | $89.00 | |||
| 718704 | 25 µg | $176.00 | |||
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E-Selectin, an adhesion molecule, belongs to the selectin/LECAM family. E-Selectin consists of one Lectin domain and several EGF-like repeats, which are followed by a transmembrane region and a short cytoplasmic tail of 32 amino acids. E-Selectin is only expressed in endothelial cells. Upon activation by TNF-α, IL-1, and LPS, E-Selectin is upregulated at the transcriptional level and reaches the maximal peak at the cell surface in three to six hours. Then the expression level returns to the baseline after 16 to 24 hours. In contrast with P-Selectin, IL-4 suppresses the expression of E-Selectin. E-Selectin binds to its ligand ESL-1, which is a glycoprotein that is expressed in neutrophils. In addition, E-Selectin binds to PSGL-1, which interacts with P-Selectin as well. The interactions between E-Selectin and its ligands initiate cell contact and leukocyte rolling on endothelial cells’ surfaces during inflammation. E-Selectin’s expression is elevated in several types of cancer cells and contributes to cancer metastasis. Thus, in Selectin-deficient mice, metastatic breast cancer cell homing to the lung vasculature is reduced.
Product Details
- Source
- Human E-Selectin, amino acids (Trp22-Pro556) (Accession# P16581), with a C-terminal human IgG1 Fc and 6-His tag was expressed in CHO cells.
- Molecular Mass
- The 774 amino acid recombinant protein has a predicted molecular mass of approximately 85.56 kD. The DTT-reduced and non-reduced protein migrates at approximately 100 and 200 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Trp.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 15 - 60 ng/mL as measured by the ability of immobilized protein to support the adhesion of U937 cells. Calcein-AM was used to measure the number of adherent cells.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Adhesion molecule.
- Distribution
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Endothelial cells.
- Function
- E-Selectin mediates leukocyte rolling during inflammation.
- Interaction
- Neutrophils and monocytes.
- Ligand/Receptor
- P-selectin glycoprotein ligand-1 (PSGL-1) and ESL-1.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Vestweber D, Blanks JE. 1999. Physiol. Rev. 79:181.
2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238.
3. Pober JS, et al. 1987. J. Immunol. 138:3319.
4. Thornhill MH, Haskard DO. 1990. J. Immunol. 145:865.
5. Asa D, et al. 1995. J. Biol. Chem. 270:11662.
6. Fries JW, et al. 1993. Am. J. Pathol. 143:725.
7. Hiratsuka S, et al. 2011. Proc. Natl. Acad. USA 108:3725.
8. Tanaka T, et al. 2011. Adv. Mater. 36:H278-82. - Gene ID
- 6401 View all products for this Gene ID
- UniProt
- View information about E-Selectin on UniProt.org