- Regulatory Status
- RUO
- Other Names
- Fibroblast growth factor 1 (FGF-1), Acidic fibroblast growth factor (aFGF), Endothelial cell growth factor (ECGF), Heparin-binding growth factor 1 (HBGF-1)
-
NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 599202 | 10 µg | $89.00 | |||
| 599204 | 25 µg | $171.00 | |||
| 599206 | 100 µg | $347.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
FGF-1, one of the most studied members of the fibroblast growth factor family, is a powerful mitogen exhibiting strong action on many different cell types. FGF-1 activity can be mediated not only by autocrine/paracrine pathways but also by an intracrine pathway. FGF-1 lacks a secretion signal peptide and is exported through a non-classical pathway. Endogenous FGF-1 is found in the nucleus of most cell types. Nuclear localization is required for FGF-1 mitogenic activity. FGF-1 promotes tumor development by promoting cancer cell proliferation and survival. Increased FGF-1 expression in early stages of many different cancers has been reported. MCF-7 breast cancer cell line overexpressing FGF-1 can form vascularized, metastatic tumors when injected into ovariectomized or tamoxifen-treated nude mice. FGF-1 also induces angiogenesis in vitro and in vivo. Thus, FGF-1 is an attractive candidate for cancer immunotargeting. FGF-1 is also involved in neuronal cell differentiation and survival. FGF-1 is highly expressed in motor neurons. In response to damage, motor neurons can release FGF-1 which results in astrocyte activation. Under oxidative stress, astrocytes can also release FGF-1 which stimulates ApoE/HDL generation in an autocrine manner for protection of the brain against oxidative stress. Involvement of FGF-1 in inflammation, cardioprotection, wound healing, adipocyte remodeling, and restenosis is also reported.
Product Details
- Source
- Human FGF-1, amino acids Phe16 - Asp155 (Accession# NP_000791) with an N-terminal Met, was expressed in E. coli.
- Molecular Mass
- The 140 amino acid recombinant protein has a predicted molecular mass of approximately 16 kD. The DTT-reduced and non-reduced protein migrates at approximately 17 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
- Purity
- >97%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 20 mM MOPS, 100 mM NaCl, pH 7.0.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 is 0.2 - 1.0 ng/ml, corresponding to a specific activity 1.0 - 5.0 x 106 units/mg, as determined by a dose-dependent stimulation of NIH3T3 cell proliferation in the presence of 10 µg/ml heparin.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human, mouse, cow, pig, rat, shark, squid, hamster, hagfish, and leech.
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Growth factor
- Distribution
-
FGF-1 is widely expressed in developing and adult tissues.
- Function
- FGF-1 plays important roles in many biological processes, including cell proliferation, inflammation, and angiogenesis. FGF-1 has low stability and a very short half-life in vivo. Binding to heparin increases the stability of FGF-1 and is also important for the formation of active FGF-acidic/FGFR complex.
- Interaction
- Intracellular FGF-1 can interact directly with CK2, FIBP, mortalin, and the ribosome-binding protein p34/leucine-rich repeat containing 59 (LRRC59).
- Ligand/Receptor
- FGF-1 can signal through all known cell-surface FGFR isoforms (FGFR1b, 1c, 2b, 2c, 3b, 3c, and 4). FGF-1 also binds to heparin and heparin sulfate proteoglycans on cell surface.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Preta M, et al. 2005. Cytokine Growth Factor Rev. 16:159.
2. Zakrzewska M, et al. 2008. Crit. Rev. Clin. Lab. Sci. 45:91.
3. Zhang L, et al. 1997. Oncogene. 15:2093.
4. Lin YZ, et al. 1996. J. Biol. Chem. 271:5305.
5. Culajay JF, et al. 2000. Biochemistry. 39:7153.
6. Cassina P, et al. 2005. J. Neurochem. 93:38.
7. Pehar M, et al. 2005. Neurodegener. Dis. 2:139.
8. Jonker JW, et al. 2012. Nature. 485:391.
9. Compagni A, et al. 2000. Cancer Res. 60:7163. - Gene ID
- 2246 View all products for this Gene ID
- UniProt
- View information about FGF-1-acidic on UniProt.org