- Regulatory Status
- RUO
- Other Names
- Fibroblast Growth Factor-10 (FGF-10), Keratinocyte growth factor 2 (KGF-2)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 710704 | 25 µg | $253.00 | |||
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FGF-10, also named keratinocyte growth factor 2 (KGF-2), is a member of the fibroblast growth factor (FGF) family and is functionally similar to the other FGF family member FGF-7. FGF-10 acts as a paracrine growth factor and plays an important role in embryonic development, cell growth, and cell differentiation. During embryonic stages, FGF-10 is essential for lung development, promoting lung morphogenesis, branching, and airway extension. FGF-10 was revealed to participate in wound healing, promoting growth of epithelial cells in normal and wounded tissue. Increased expression of FGF-10 and the receptor FGFR2-IIIb has been reported to associate with various malignant diseases, such as prostate, breast, pancreas, and colorectum cancer, suggesting the role of FGF-10 in carcinogenesis.
Product Details
- Source
- Human FGF-10, amino acids (Leu40 - Ser208) (Accession# NP_004456.1) with N-terminal Met, was expressed in E. coli.
- Molecular Mass
- The 170 amino acid recombinant protein has a predicted molecular mass of approximately 19.3 kD. The predicted N-terminal amino acid is Met.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE gel and HLPC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in 5 mM sodium phosphate, pH 7.4 to a concentration of 0.1-1.0 mg/ml. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg, as measured by its ability to stimulate thymidine uptake by BaF3 cells expressing FGF receptors in a dose dependent manner.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and mouse.
Antigen Details
- Structure
- Growth factor
- Distribution
-
FGF-10 is a secretary protein, expressed and secreted by the mesenchyme fibroblasts during embryonic development.
- Function
- FGF-10 is a growth factor required for brain development, lung development, and lung morphogenesis during embryonic stages. FGF-10 was shown to play an important role in wound healing.
- Interaction
- FGF-10 targets urothelial cells, epithelial cells, epidermal cells, keratinocyte, and smooth muscle cells. FGF-10 interacts with FGFBP1.
- Ligand/Receptor
- FGFR2-IIIb.
- Bioactivity
- Human FGF-10 is able to induce proliferation of BaF3 cells expressing FGFR.
- Cell Type
- Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Sugimoto K, et al. 2014. Genes Cells. 19:112.
2. McQualter JL, et al. 2013. Stem Cell Res. 11:1222.
3. Benjamin JT, et al. 2010. J. Immunol. 185:4896.
4. Chioni AM, et al. 2009. Eur. J. Cell Biol. 88:371.
5. Zhang D, et al. 2006. Am. J. Physiol. Renal Physiol. 291:481.
6. Beer HD, et al. 2005. Oncogene. 24:5269. - Gene ID
- 2255 View all products for this Gene ID
- UniProt
- View information about FGF-10 on UniProt.org