- Regulatory Status
- RUO
- Other Names
- Fibroblast growth factor 8 (FGF8), Androgen-induced growth factor (AIGF), Heparin-binding growth factor 8 (HBGF-8)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 710504 | 25 µg | $253.00 | |||
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FGF-8 belongs to the fibroblast growth factor (FGF) family and plays an important role in cell growth, embryogenesis, and tumorigenesis. Four isoforms (a, b, e, and f) of human FGF-8 generated by alternative splicing of the mRNA have been described. The isoforms may have different functions during embryogenesis. During prenatal stages, FGF-8 is required for the normal development of various organs, including limbs and central nervous system. FGF-8 was first cloned from SC-3 mouse mammary carcinoma cells and was found to be induced in response to androgenic stimuli. FGF-8 is found to have a transforming capacity and is detected in human prostate and breast carcinoma specimens and cell lines. The isoform FGF-8b has the highest affinity to FGF receptors and the strongest transforming capacity.
Product Details
- Source
- Human FGF-8 isoform b, amino acids (Gln23 - Arg215) (Accession# NP_006110.1) with N-terminal Met, was expressed in E. coli.
- Molecular Mass
- The 194 amino acid recombinant protein has a predicted molecular mass of approximately 22.5 kD. The predicted N-terminal amino acid is Met.
- Purity
- >97%, as determined by Coomassie stained SDS-PAGE gel and HLPC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Concentration
- This product is bottled at the concentration indicated on the vial.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 2.0 - 4.0 ng/ml, corresponding to a specific activity of 2.5 - 5.0 x 105 units/mg,as measured by its ability to stimulate proliferation of BALB/c 3T3 cells in a dose dependent manner.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and mouse.
Antigen Details
- Structure
- Growth factor
- Distribution
-
Secreted protein; widely expressed during embryonic stages; low expression levels in adult hematopoietic cells and gonads.
- Function
- FGF-8 is involved in the regulation of cell proliferation and cell differentiation during embryonic development.
- Interaction
- Embryonic progenitor cells.
- Ligand/Receptor
- FGFR1, FGFR2, FGFR3 and FGFR4.
- Bioactivity
- Human FGF-4 is able to induce proliferation of Balb/c 3T3 cells.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Falardeau J, et al. 2008. J. Clin. Invest. 118:2822.
2. Turner N, et al. 2010. Nat. Rev. Cancer. 10:116.
3. Tanaka S, et al. 2001. Dig. Dis. Sci. 46:1016.
4. Payson RA, et al. 1996. Oncogene. 13:47.
5. Gemel J, et al. 1996. Genomics. 35:253.
6. Han MJ, et al. 2001. Dev Dyn. 220:40. - Gene ID
- 2253 View all products for this Gene ID
- UniProt
- View information about FGF-8 on UniProt.org