- Regulatory Status
- RUO
- Other Names
- Fibroblast Growth Factor-9, Glia-activating factor (GAF), Heparin-binding growth factor 9 (HBGF-9)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 710604 | 20 µg | $253.00 | |||
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FGF-9 is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family. FGF-9 was first identified as a secretory protein from the human glioma cell line NMC-G1 and is reported to be widely expressed in rat brain. In the central nervous system, FGF-9 acts as an autocrine and a paracrine growth factor that is required for the development, growth, and survival of neuronal cells. During prenatal stages, FGF-9 expression is crucial for normal lung and airway development. FGF-9 null mutant mice show the phenotype of severe lung hypoplasia, which results in perinatal death. FGF-9 is key in stimulating cell proliferation after arterial, skeletal, and hepatic injuries. FGF-9 is also involved in tumorgenesis, enhancing transformation and progression of several malignant diseases, including prostate, lung, and colon cancer.
Product Details
- Source
- Human FGF-9, amino acids (Pro3 - Ser208) (Accession# NP_002001.1), was expressed in E. coli.
- Molecular Mass
- The 206 amino acid recombinant protein has a predicted molecular mass of approximately 23.2 kD. The predicted N-terminal amino acid is Pro.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE gel and HLPC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Concentration
- This product is bottled at the concentration indicated on the vial.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg, as measured by its ability to stimulate thymidine uptake by BaF3 cells expressing FGF receptors in a dose dependent manner.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and mouse.
Antigen Details
- Structure
- Growth factor
- Distribution
-
FGF-9 is a secreted protein, widely expressed in central nervous system. FGF-9 is expressed in lung mesothelium and epithelium during organogenesis.
- Function
- FGF-9 is a growth factor and is involved in embryonic development, cell growth, cell differentiation, cell migration, and carcinogenesis. FGF-9 is required for the development of glial cells in nervous system.
- Interaction
- FGF-9 targets glial cells in nervous system.
- Ligand/Receptor
- FGFR1-IIIc, FGFR2-IIIc, FGFR3-IIIb, FGFR3-IIIc, and FGFR4.
- Bioactivity
- Human FGF-9 is able to induce proliferation of BaF3 cells expression FGFR.
- Cell Type
- Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Reuss B, et al. 2003. Cell Tissue Res. 313:139.
2. Tsai SJ, et al. 2002. Endocrinology. 143:2715.
3. White AC, et al. 2006. Development. 133:1507.
4. Workalemahu G, 2004. J. Leukoc. Biol. 75:657.
5. Teishima J, 2014. Anticancer Res. 34:695.
6. Behr B, 2010. Proc. Natl. Acad. Sci. USA. 107:11853. - Gene ID
- 2254 View all products for this Gene ID
- UniProt
- View information about FGF-9 on UniProt.org