- Regulatory Status
- RUO
- Other Names
- Fibroblast growth factor 2 (Fgf-2), Fgfb, bFGF
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 713304 | 50 µg | $253.00 | |||
This product is not available for shipping outside of the United States.
FGF-basic, also known as FGFb and FGF-2, is a member of the fibroblast growth factor (FGF) family which includes 23 members. FGFb is expressed in almost all tissues and plays an important role in a variety of normal and pathological processes, including development, wound healing, and neoplastic transformation. FGFb is mitogenic for many cell types, both epithelial and mesenchymal. FGFb shows potent angiogenic activity and has been implicated in tumor angiogenesis. In prostate, bladder, and renal cancers, FGFb regulates the induction of metalloproteinases (MMP) that degrade extracellular matrix proteins, thus facilitating tumor metastasis. FGFb binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to -4. In addition, FGFb binds to the ECM, and heparan sulfate (HS) is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface, although there is controversy regarding the exact manner by which this occurs.
Product Details
- Source
- Human FGF-basic, amino acids Pro143-Ser288 (Accession# NM_002006) was expressed in E. coli.
- Molecular Mass
- The 146 amino acid recombinant protein has a predicted molecular mass of approximately 16.4 kD. The predicted N-terminal amino acid is Pro.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg as determined by a cell proliferation assay using BALB/c 3T3 cells.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and mouse.
- Product Citations
-
Antigen Details
- Distribution
- Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, and epithelial cells
- Function
- FGFb is a potent angiogenic factor, and plays a key role in various physiological and pathological conditions, including embryonic development, wound repair, inflammation, and tumor growth.
- Interaction
- Fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, astrocytes, oligodendrocytes, and smooth muscle cells.
- Ligand/Receptor
- FGFR-1 (flg), FGFR-2 (bek, K-sam), FGFR3, and FGFR-4 (flg-2); low affinity coreceptor heparin sulfate and heparin sulfate proteoglycans required for full activity.
- Bioactivity
- Stimulation of 3T3 cell proliferation.
- Cell Type
- Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Rusnati M and Presta M. 2007. Current Pharm. Des. 13:2025.
2. Chaffer CL, et al. 2007. Differentiation 75:831.
3. Cronauer NV, et al. 2003. Eur. Urol. 43:309.
4. Shimizu A, et al. 2001. J. Biol. Chem. 276:11031.
5. Mahammadi M, et al. 2005. Curr. Opin. Struct. Biol. 15:506.
6. Chiou M and Xu Y. 2006. Longaker MT Biochem. Biophys. Res. Commun. 343:644. - Gene ID
- 2247 View all products for this Gene ID
- UniProt
- View information about FGF-basic on UniProt.org