- Regulatory Status
- RUO
- Other Names
- Fibroblast growth factor 2 (FGF-2), FGF-β, Basic fibroblast growth factor (bFGF)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 710304 | 50 µg | $253.00 | |||
| 710308 | 500 µg | $528.00 | |||
This product is not available for shipping outside of the United States.
FGF-2, also known as FGF-β, is a member of the fibroblast growth factor (FGF) family, which includes 23 members. FGF-2 is expressed in almost all tissues and plays an important role in a variety of normal and pathological processes, including development, wound healing, and neoplastic transformation. FGF-2 is mitogenic for many cell types, both epithelial and mesenchymal. It shows potent angiogenic activity and has been implicated in tumor angiogenesis. FGF-2 significantly promotes the proliferation of adipose-derived mesenchymal cells (AMC) and enhances chondrogenesis in three-dimensional micromass culture. FGF-2 binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to -4. In addition, FGF-2 binds to the extracellular matrix (ECM) and heparan sulfate (HS), and is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enable FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface, although there is controversy regarding the exact manner by which this occurs.
Product Details
- Source
- Human FGF-2, amino acids (Ala135 - Ser288) (Accession# NP_001997.5), was expressed in E. coli.
- Molecular Mass
- The 154 amino acid recombinant protein has a predicted molecular mass of approximately 17.2 kD. The predicted N-terminal amino acid is Ala.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE gel and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.01 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as measured by its ability to stimulate proliferation of BALB/c 3T3 cells in a dose dependent manner.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human, cow, dog, frog, hamster, monkey, mouse, pig, rabbit, rat, and sheep.
- Product Citations
-
Antigen Details
- Structure
- Growth factor
- Distribution
-
Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, and epithelial cells.
- Function
- FGF-2 is a potent angiogenic factor, and plays a key role in various physiological and pathological conditions, including embryonic development, wound repair, inflammation, and tumor growth.
- Interaction
- Fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, astrocytes, oligodendrocytes, and smooth muscle.
- Ligand/Receptor
- FGFR-1 (flg), FGFR-2 (bek, K-sam), FGFR3, and FGFR-4 (flg-2); low affinity coreceptor heparin sulfate and heparin sulfate proteoglycans required for full activity.
- Bioactivity
- Human FGF-2 is able to stimulate proliferation of Balb/c 3T3 cells.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 2247 View all products for this Gene ID
- UniProt
- View information about FGF-2 on UniProt.org