- Regulatory Status
- RUO
- Other Names
- Fms-related tyrosine kinase 3 ligand, SL cytokine, Flt3 Ligand
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 710802 | 10 µg | $253.00 | |||
This product is not available for shipping outside of the United States.
Fms-like tyrosine kinase 3 ligand is a hematopoietic cytokine structurally similar to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). Flt-3 ligand synergizes with other growth factors, such as interleukin-7 (IL-7) and CSFs, to promote proliferation and differentiation of early hematopoietic cells. In addition, Flt-3 ligand is involved in the commitment and expansion of dendritic cells as well as stimulating B cells, T cells, and NK cells. Both soluble form and membrane-bound forms of Flt-3 ligand are able to stimulate the cells expressing receptor tyrosine kinases Flt-3, transducing signals through the ERK and PI3K pathway. Flt-3 ligand is the only known ligand for Flt-3. Flt-3 and Flt-3 ligand have been implicated to participate in leukemogenesis, since the constitutively activated mutants of Flt-3 were found to be associated with the acute myeloid leukemia (AML).
Product Details
- Source
- Human Flt-3 Ligand, amino acids (Thr27 - Ala181) (Accession# NP_004110.2) was expressed in E. coli.
- Molecular Mass
- The 155 amino acid recombinant protein has a predicted molecular mass of approximately 17.6 kD. The predicted N-terminal amino acid is Thr.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Concentration
- This product is bottled at the concentration indicated on the vial.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg, as measured by its ability to stimulate the proliferation of human AML5 cells in a dose dependent manner.
- Application
-
Bioassay
- Product Citations
-
Antigen Details
- Structure
- Growth factor
- Distribution
-
Flt-3 ligand is expressed by hematopoietic cells and bone marrow stromal cells in both secreted and membrane bound form.
- Function
- Flt-3 ligand is a growth factor, stimulating differentiation, proliferation, and survival of hematopoietic progenitor cells.
- Interaction
- FLT-3 ligand targets myeloid progenitor cells, lymphoid progenitor cells, B cells, T cells, NK cells, and dendritic cells.
- Ligand/Receptor
- Flt-3
- Bioactivity
- Human Flt-3L is able to induce proliferation of AML5 cells.
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Ding Y, et al. 2014. J. Immunol. 192:1982.
2. Kreiter S, et al. 2011. Cancer Res. 71:6132.
3. Zheng R, et al. 2011. Oncogene 30:4004.
4. Rahman S, et al. 2011. J. Immunol. 186:390.
5. Sathaliyawala T, et al. 2010. Immunity 33:597.
6. Drexler HG and Quentmeier H, et al. 2004. Growth Factors 22:71. - Gene ID
- 2322 View all products for this Gene ID
- UniProt
- View information about FLT3L on UniProt.org