- Regulatory Status
- RUO
- Other Names
- Granulocyte-Colony Stimulating Factor, CSF3, CSF-β, Pluripoietin, Granulocytic neutrophil colony stimulating activity (G-CSA), Macrophage-granulocyte inducer-2 (MGI-2)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 713402 | 10 µg | $253.00 | |||
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Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil development. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9. This in turn degrades important HSC trafficking and adhesion molecules, such as c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T cells. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.
Product Details
- Source
- Human G-CSF, amino acids Thr27-Pro200 (Accession# BC033245.1), was expressed in E.coli.
- Molecular Mass
- The 174 amino acid recombinant protein has a predicted molecular mass of 18.6 kD. The predicted N-terminal amino acid is Thr.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg as determined by a cell proliferation assay using mouse NFS-60 cells.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and mouse.
- Product Citations
-
Antigen Details
- Distribution
- Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, epithelial cells, and bone marrow stromal cells.
- Function
- G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxide anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
- Interaction
- Hematopoietic progenitor cells, neutrophils, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, cardiac fibroblasts, neurons, and some small cell lung carcinoma cell lines.
- Ligand/Receptor
- CD114 (G-CSFR)
- Bioactivity
- Stimulation of mouse NFS-60 cells
- Cell Type
- Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Nagata S, et al. 1986. Nature 319:415.
2. Kim HK, et al. 2006. Blood 108:812.
3. Donahue RE, et al. 2009. Blood 114:2530.
4. Shimoji K, et al. 2010. Cell Stem Cell 6:227.
5. Martins A, et al. 2010. IUBMB Life 62:611.
6. Hara M, et al. 2011. J. Exp. Med. 208:715. - Gene ID
- 1441 View all products for this Gene ID
- UniProt
- View information about G-CSF on UniProt.org