- Regulatory Status
- RUO
- Other Names
- Auxillin-2, Auxillin 2, Auxillin II, cyclin G associated kinase, cyclinG-associated kinase, EC 2.7.11.1, GAK, DNAJ26
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Stability Testing for Recombinant Human GAK. One aliquot (Control) was stored at 4°C for 7 days, while another aliquot (4XF/T) was subjected to multiple freeze-thaw cycles by freezing at -80°C and thawing four times over a 7-day period. Each freeze-thaw cycle involved placing the sample on ice and slowly thawing it until no ice crystals remained in the tube. A minimum of 24 hours elapsed between each freeze and thaw event. Following treatment, the reduced samples (4 µg/lane) were separated by SDS-PAGE and stained with Coomassie brilliant blue dye. Lane M: Protein ladder (Cat. No. 773302)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 568906 | 100 µg | $300.00 | |||
Cyclin G-associated kinase (GAK), also known as auxilin 2, is a serine/threonine protein kinase that plays a crucial role in cellular processes, including membrane trafficking and cell cycle regulation. GAK contains distinct domains, including a Ser/Thr protein kinase domain, a phosphatase tensin/auxilin domain, a PTEN C2 domain, and a J domain. GAK is widely expressed in various cells and tissues. GAK plays a key role in clarthrin-mediated endocytosis via the interaction of the heat shock cognate protein 70. GAK regulates cyclin-dependent kinases. GAK is also involved in the regulation of protein degradation pathways, including the ubiquitin-proteasome system. GAK has emerged as a gene associated with Parkinson's disease.
Product Details
- Source
- Human GAK, amino acids (Gly13-Val351) (Accession# O14976-1) with a N-terminal 6His tag, was expressed in insect cells
- Molecular Mass
- The 352 amino acid recombinant protein has a predicted molecular mass of approximately 39.72 kD. The DTT-reduced and non-reduced protein migrates at approximately 31-50 kD by SDS-PAGE.
- Purity
- > 85% as determined by Coomassie stained SDS-PAGE.
- Formulation
- Protein is formulated in 50 mM HEPES, 150 mM NaCl, 25% glycerol, 1mM TCEP, pH 7.5
- Concentration
- 100 µg at 0.5 mg/mL
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 1 week, at -20°C for up to three months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Application
-
Standard
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped with dry ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
Broadly distributed in normal adult tissues including brain, Lymphoid, lung, gastrointestinal tract, kidney, female reproduction system, and kidney
- Function
- Clathrin-mediated endocytosis, Membrane Trafficking, Vesicle-mediated transport
- Interaction
- Androgen receptor
- Cell Type
- Endothelial cells, Epithelial cells, Lymphocytes, Mesenchymal cells
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Neurodegeneration, Neuroscience, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase, Synaptic Vesicle Trafficking/Endocytosis
- Antigen References
-
- Shimizu H, et al. 2009. J Cell Sci. 122:3145-52.
- Tanenbaum ME, et al. 2010. Chromosoma. 119:415-24.
- Dong-Won L, et al. 2005. J Cell Sci. 118:4311.
- Dong-Won L, et al. 2006. J Cell Sci. 119:3502.
- Pickrell JK, et al. 2016. Nat Genet. 48: 709-17.
- Yang-In Y, et al. 2010. Proc Natl Acad Sci USA. 76:89-92.
- Gene ID
- 2580 View all products for this Gene ID
- UniProt
- View information about GAK on UniProt.org