- Regulatory Status
- RUO
- Other Names
- Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor (ATF)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 711002 | 10 µg | $253.00 | |||
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Glial cell line-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor Ret receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, including depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease, since GNDF can protect dopaminergic neurons from neurotoxin and promotes neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and development of kidney. Upregulated expression of GNDF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.
Product Details
- Source
- Human GDNF, amino acids (Ser78 - Ile211) (Accession# NP_000505.1) with an N-terminal Met, was expressed in E. coli.
- Molecular Mass
- The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.0 kD. Recombinant human GDNF forms a a disulfide-linked homodimer. The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store in working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as measured by its ability to stimulate proliferation of rat C6 cells in a dose dependent manner.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human, mouse, and monkey.
- Product Citations
-
Antigen Details
- Structure
- Growth factor
- Distribution
- GDNF is a secreted protein and is widely expressed in the developing and adult central nervous system.
- Function
- GDNF is a neurotrophic factor, promoting the development, differentiation, and survival of dopaminergic neurons. GNDF participates in the recovery processes after brain injury.
- Interaction
- GDNF targets the neuronal cells expressing GDNF family receptors.
- Ligand/Receptor
- GDNF interacts with GDNF family receptor α-1 (GFRα1). Lower affinity with GFRα2, GFRα3, and GFRα4.
- Bioactivity
- Recombinant human GDNF is able to induce proliferation of rat C6 cells.
- Cell Type
- Neural Stem Cells, Embryonic Stem Cells
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Allen SJ, et al. 2013. Pharmacol. Ther. 138:155.
2. Hendrich J, et al. 2012. Neuroscience. 219:204.
3. Hidalgo-Figueroa M, et al. 2012. J. Neurosci. 32:864.
4. Pascual A, et al. 2011. J. Mol. Endocrinol. 46:83.
5. Carnicella S, et al. 2009. Pharmacol. Ther. 122:9.
6. Sariola H, et al. 2003. J. Cell. Sci. 116:3855. - Gene ID
- 2668 View all products for this Gene ID
- UniProt
- View information about GDNF on UniProt.org