- Regulatory Status
- RUO
- Other Names
- Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor (ATF), ATF1, ATF2, HSCR3, HFB1-GDNF
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When human GDNF is immobilized at 0.5 µg/mL, human GFRα1 binds with EC50 of 10 - 50 ng/mL in a functional ELISA.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 760401 | 2 µg | $89.00 | |||
| 760402 | 10 µg | $223.00 | |||
This product is not available for shipping outside of the United States.
Glial cell line-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor Ret receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, which include depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease because it can protect dopaminergic neurons from neurotoxins and promotes neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and kidney development. Upregulated expression of GDNF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.
Product Details
- Source
- Human GDNF, amino acids (Ser78-Ile211) (Accession# NP_000505.1) with N-terminal Met, was expressed in E. coli.
- Molecular Mass
- The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.0 kD. The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by SDS-PAGE gel and HPLC analysis.
- Formulation
- Lyophilized from 0.2 µm filtered protein solution in 10 mM Sodium Citrate and 150 mM NaCl.
- Endotoxin Level
- Less than 1.0 EU per µg protein as determined by the LAL method.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1 - 0.5 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- EC50 = 10 - 50 ng/mL as measured by the ability of the immobilized protein binding to GFRα1 in a functional ELISA.
- Application
-
Bioassay
Antigen Details
- Structure
- Disulfide-linked homodimer.
- Distribution
- GDNF is a secreted protein that is widely expressed in the developing and adult central nervous system.
- Function
- GDNF is a neurotrophif factor that promotes the development, differentiation, and the survival of dopaminergic neurons. It also participates in the recovery process after brain surgery.
- Interaction
- GDNF targets the neuronal cells expressing GDNF family receptors.
- Ligand/Receptor
- GDNF interacts with GDNF family receptor α-1 (GFRα1) and has a lower affinity with GFRα2, GFRα3, and GFRα4.
- Bioactivity
- Measured by the ability of immobilized human GDNF to bind to GFRα1 in a functional ELISA.
- Cell Type
- Neural Stem Cells, Embryonic Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors
- Antigen References
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1. Allen SJ, et al. 2013. Pharmacol. Ther. 138:155.
2. Hendrich J, et al. 2012. Neuroscience 219:204.
3. Hidalgo-Figueroa M, et al. 2012. J. Neurosci. 32:864.
4. Pascual A, et al. 2011. J. Mol. Endocrinol. 3:R83-92.
5. Carnicella S, et al. 2009. Pharmacol. Ther. 122:9.
6. Sariola H, et al. 2003. J. Cell Sci. 116:3855. - Gene ID
- 2668 View all products for this Gene ID
- UniProt
- View information about GDNF on UniProt.org