- Regulatory Status
- RUO
- Other Names
- Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 713604 | 20 µg | $253.00 | |||
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GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRα) and a major signaling subunit (βc). The receptor subunits are always coexpressed on the surface of leukocytes, with βc being expressed at lower levels than GMRα. Certain non-hemopoietic cell types have also been reported to express the GM-CSF receptor and respond to GM-CSF stimulation in vitro, although the in vivo significance of these observations remains uncertain.
Product Details
- Source
- Human GM-CSF, amino acids Ala18- Glu144 (Accession# NM_000758), was expressed in E. coli.
- Molecular Mass
- The 128 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.5 kD. The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
- Application
-
Bioassay
- Product Citations
-
Antigen Details
- Structure
- Cytokine
- Distribution
- T cells, monocytes, macrophages, fibroblasts, endothelial cells, mast cells
- Function
- Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in the extracellular matrix with heparan sulfate proteoglycans.
- Interaction
- Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts.
- Ligand/Receptor
- Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common.
- Bioactivity
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) and the related cytokines IL-3 and IL-5 regulate the production and functional activation of hematopoietic cells, with GM-CSF acting on monocytes, macrophages and all granulocytes.
- Cell Type
- Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Hercus TR, et al. 2009. Blood 114:1289.
2. Hayashida K, et al. 1990. Proc Natl Acad Sci USA 87:9655.
3. Walker F, et al. 1985. EMBO J. 4:933.
4. Kitamura T, et al. 1989. J. Cell Physiol. 140:323. - Gene ID
- 1437 View all products for this Gene ID
- UniProt
- View information about GM-CSF on UniProt.org