- Regulatory Status
- RUO
- Other Names
- Neuregulin-1 (NRG-1), Acetylcholine receptor-inducing activity (ARIA), Neu differentiation factor (NDF)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 711104 | 50 µg | $253.00 | |||
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Heregulin, also named neuregulin-1 type I, is a family of growth factors that act as ligands for the epidermal growth factor receptors, including ERBB3 and ERBB4. Heregulins regulate survival, proliferation, and differentiation of the cells during development and wound healing. Heregulin-β1 is one of the Heregulin isoforms derived from alternative splicing. Binding of Heregulin-β1 to receptor tyrosine kinases ERBB3 or ERBB4 results in the recruitment and activation of ERBB2, subsequently transducing signaling cascades through the mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3 kinase (PI3K) pathways. It has been suggested that heregulin-β1 participates in the tumorgenesis and metastasis of breast cancer, promoting proliferation, migration, and invasion of breast cancer cells.
Product Details
- Source
- Human Heregulin-β1, amino acids (Ser177-Glu241) (Accession# NM_013956), was expressed in E. coli.
- Molecular Mass
- The 65 amino acid recombinant protein has a predicted molecular mass of approximately 7.5 kD. The predicted N-terminal acid is Ser.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store in working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg, as measured by its ability to stimulate proliferation of human MCF7 cells in a dose dependent manner.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human, rat, and mouse.
- Product Citations
-
Antigen Details
- Structure
- Growth factor
- Distribution
- Heregulin-β1 is a secreted protein and is expressed majorly in neuronal cells.
- Function
- Heregulin-β1 is the ligand for ERBB3 and ERBB4 tyrosine kinase receptors, modulating cell growth and differentiation. Heregulin-β1 has been shown to promote invasiveness and metastasis of breast tumor cells.
- Interaction
- Heregulin-β1 targets a variety of cell types, including epithelial, glial, neuronal, and skeletal muscle cells.
- Ligand/Receptor
- Heregulin-β1 interacts with ERBB3 and ERBB4.
- Bioactivity
- Human Heregulin-β1 is able to induce proliferation of MCF-7 cells.
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Synaptic Biology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Kim S, et al. 2012. Exp. Mol. Med. 44:473.
2. Hutcheson IR, et al. 2011. Breast Cancer Res. 13:R29.
3. Pan MH, et al. 2011. Evid. Based Complement Alternat. Med. 2011:562187.
4. Hernandez L, et al. 2009. Cancer Res. 69:3221.
5. Hutcheson IR, et al. 2007. Breast Cancer Res. 9:R50.
6. Thottassery JV, et al. 2004. Cancer Res. 64:4637. - Gene ID
- 3084 View all products for this Gene ID
- UniProt
- View information about Heregulin-beta1 on UniProt.org