- Regulatory Status
- RUO
- Other Names
- Interferon lambda-1, Interleukin-29 (IL-29)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 711204 | 20 µg | $253.00 | |||
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IFN-λ1, also known as IL-29, is a member of the type III interferon (IFN) family. Type III interferons, including IFN-λ1, IFN-λ2 and IFN-λ3, are produced upon virus infection and are functionally similar to type I interferon (IFN-α/β). IFN-λ binds to a heterodimeric receptor composed of the IFN-λ receptor 1 (IFNLR1) and the interleukin-10 receptor 2 (IL-10R2), subsequently tranducing signal through the JAK-STAT pathway and leading to transactivation of various IFN-stimulated genes (ISGs). IFN-λ has been demonstrated to mediate an antiviral immune response against several viruses, such as hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). In contrast to type I or type II interferons, IFN-λ receptor is expressed preferentially on epithelial-like cells and some immune cells. Because the response to IFN-λ is highly restricted to certain cell types, IFN-λ is considered a potential therapuetic agent for the treatment of viral diseases with less side effects when compared with type I interferon.
Product Details
- Source
- Human IFN-λ1, amino acids (Pro23-Thr200) (Accession# NP_742152.1), was expressed in E. coli.
- Molecular Mass
- The 178 amino acid recombinant protein has a predicted molecular mass of approximately 19.8 kD. The predicted N-terminal amino acid is Pro.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.2 - 0.5 ng/ml, corresponding to a specific activity of 2.0 x 106 - 5.0 x 106 units/mg, as measured by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and monkey.
- Product Citations
-
Antigen Details
- Structure
- Cytokine
- Function
- IFN-λ1 is responsible for mediating an immune response against viral pathogens. Expression of IFN-λ1 is induced by double stranded RNA or virus infection.
- Interaction
- IFN-λ1 targets epithelial cells and hepatocytes, which express the IFN-λ receptor.
- Ligand/Receptor
- IFN-λ1 interacts with the heterodimeric cytokine receptor composed of IL10R2 and IFNLR1.
- Bioactivity
- Human IFN-λ1 is able to induce STAT phosphorylation.
- Biology Area
- Immunology
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Swider A, et al. 2014. Cytokine. 65:17.
2. Rauch I, et al. 2013. JAKSTAT 2:e23820.
3. Lasfar A, et al. 2011. Clin. Dev. Immunol. 2011:349575.
4. Donnelly RP, et al. 2011. Trends Immunol. 32:443.
5. Kelly C, et al. 2011. Gut. 60:1284.
6. Donnelly RP and Kotenko SV. 2010. J. Interferon. Cytokine Res. 30:555. - Gene ID
- 282618 View all products for this Gene ID
- UniProt
- View information about IL-29 on UniProt.org