- Regulatory Status
- RUO
- Other Names
- Insulin-like growth factor I, somatomedin C, Nonsuppressibleinsulin-like activity I (NSILA-I), Insulin-Like Growth Factor IB, IBP1
-
Recombinant human IGF-1 induces the proliferation of MCF-7 human epithelial cells in a dose-dependent manner. The ED50 for this effect is 1.3 – 6.5 ng/mL. -
Stability Testing for Recombinant Human IGF-1. Recombinant human IGF-1 was aliquoted in 300 mM ammonium acetate, pH 5.5 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property induce the proliferation of MCF-7 human epithelial cells in a dose-dependent manner. The ED50 for this effect is 1.3 – 6.5 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 588604 | 25 µg | $55.00 | |||
| 588606 | 100 µg | $125.00 | |||
IGF-I (insulin-like growth factor 1), initially described in 1957, is a hormone that is important in childhood growth and anabolic effects in adults. It is a member of the IGF family that is comprised of three members: IGF-I, IGF-II, and insulin. IGF-I and IGF-II share approximately 50% similarity with insulin at the amino acid level. Human IGF-I is synthesized as a 191 amino acid prepro-IGF-I. Process of maturation to Pro-IGF- requires glucose regulating protein 94 (GRP94) that possesses chaperone activity towards IGF-I. Deletion of muscle GRP94 impairs muscle and body growth by inhibiting local production of IGF proteins. Seven binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half-life and regulate the availability of IGF-I and IGF-II. It has been described that IGFBP3 is the main circulating binding protein for IGFs. In serum approximately 75% of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-I binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration. IGF-I deficiency is associated to growth hormone insensitivity syndrome (GHIS) also called Laron syndrome, liver cirrhosis, age-related cardiovascular and neurological diseases, and intrauterine growth restriction.
Product Details
- Source
- Human IGF-1, amino acid sequence (Gly49-Ala118) (Accession: #P05019.1), was expressed in E.coli. The amino-terminus contains Met-6His-IEGR tag.
- Molecular Mass
- The 87 amino acid recombinant protein has a predicted molecular mass of approximately 9.6 kD. The DTT-reduced and non-reduced proteins migrate at approximately 10 kD and 12 kD respectively, by SDS-PAGE. The predicted N-terminal amino acid is Met.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 300 mM ammonium acetate, pH 5.5
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human IGF-1 induces the proliferation of MCF-7 human epithelial cells in a dose-dependent manner. The ED50 for this effect is 1.3 – 6.5 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
Astrocytes, hepatocytes, microglia, intestinal smooth muscle, myoblast, NK cells
- Function
- IGF-I induces mitogenesis, proliferation, growth, differentiation, and angiogenesis. IGF-I is a mediator of growth hormone action. IGF-I is involved in neuronal myoblast proliferation and differentiation, and organ development. IGF-I promotes NK cell development and cytotoxic activity in NK cells.
- Interaction
- Neuronal cells, myoblast, T cells, B cells, NK cells
- Ligand/Receptor
- IGF-I receptor, Insulin receptor isoform A, and IGF-II receptor (M-6-P-R)
- Bioactivity
- Human IGF-1 induces the proliferation of MCF-7 human epithelial cells.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Bell GI, et al. 1984. Nature. 310:775-7.
- Karey KP and Sirbasku DA, 1988. Cancer Res. 48:4083-92.
- Mohan S and Baylink DJ, 2002. J Endocrinol. 175:19-31.
- Ostrovsky O, et al. 2010. Biochim Biophys Acta. 1803:333-41.
- Barton ER, et al. 2012. FASEB J. 26:3691-702.
- Puche JE and Castilla-Cortázar I. 2012. J Transl Med. 10:224.
- Ni F, et al. 2013. Nat Commun. 4:1479.
- Gene ID
- 3479 View all products for this Gene ID
- UniProt
- View information about IGF-1 on UniProt.org