- Regulatory Status
- RUO
- Other Names
- Interleukin 15, Interleukin-15, IL15, MGC9721
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Recombinant human IL‐15 induces the proliferation of MO7e human megakaryocytic leukemic cell line in a dose dependent manner. BioLegend’s product was compared side‐by‐side to the leading competitor’s equivalent product.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 570302 | 10 µg | $229.00 | |||
| 570304 | 25 µg | $347.00 | |||
| 570306 | 100 µg | $891.00 | |||
| 570308 | 500 µg | $2461.00 | |||
IL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). Interleukin-15 (IL-15) is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes (2). IL-15 plays an important role in immune response and shares many functions with IL-2, for example, stimulating the proliferation of activated T cells (1, 2), NK cells (3) and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand (4). In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8). IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation (9).
Product Details
- Source
- Human IL-15, amino acids Asn49-Ser162 (Accession# NM_000585), was expressed in E. coli.
- Molecular Mass
- The 114 amino acid recombinant protein has a predicted molecular mass of 12,773 Da. The DTT-reduced protein migrates at approximately 9kDa and the non-reduced protein migrates at approximately 7kDa by SDS-PAGE. The N-terminal amino acid is Asn.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
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ED50 = 2 - 10 ng/ml, corresponding to a specific activity of 0.1 - 0. 5 x 106 units/mg, as determined by the dose dependent stimulation of MO7e cell proliferation.
The specific activity of recombinant human IL-15 is approximately 3.51 x 104 IU/µg when compared against the WHO Reference Reagent for Human Interleukin-15 (NIBSC code: 95/554) as determined by the dose dependent stimulation of MO7e cell proliferation.
For more information on specific activity, please visit the Recombinant Protein Unit Conversions page. - Application
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Bioassay
- Recommended Usage
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Use when high specific biological activity is required.
- Application Notes
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This IL-15 protein is biologically active and can be used for in vitro assays.
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com. -
Application References
(PubMed link indicates BioLegend citation) -
- Correia DV, et al. 2011. Blood 118:992. (BA) PubMed
- Product Citations
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Antigen Details
- Structure
- 13 kD single non-glycosylated polypeptide
- Distribution
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IL-15 is expressed by DC, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes.
- Function
- IL-15 stimulates the proliferation of activated T cells (1, 2), NK cells (3) and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand (4). In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8).
- Bioactivity
- stimulatory activities on the proliferation, survival and activation of T lymphocytes and NK cells, and the pathogenesis of rheumatoid arthritis via activating T cells and induces IL-17 production
- Cell Sources
- adherent peripheral blood mononuclear cells, fibroblasts and epithelial cells
- Cell Targets
- T lymphocytes, NK cells
- Receptors
- IL-15Rα, IL-15Rβ and IL-15γc
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Grabstein K, et al. Science 264:965-968 1994.
2. Ma A, et al. Annu Rev Immunol 24:657-679 2006.
3. Meresse B, et al. Immunity 21:357-366 2004.
4. Armitage RJ et al, J Immunol 154:483-490 1995.
5. Pulendran B, et al. Eur. J. Immunol. 34:66-73 2004.
6. Bouchard A, et al. J. Leukoc. Biol. 76:162-168 2004.
7. Ratthe C, et al. 2004. J. Leukoc. Biol. 75:893.
8.Feng T, et al. 2008. Cell Immunol 5:189.
9. Rubinstein MP et al, P. Natl. Acad. Sci. USA 103:9166-9171 2006. - Gene ID
- 3600 View all products for this Gene ID
- UniProt
- View information about IL-15 on UniProt.org