- Regulatory Status
- RUO
- Other Names
- Interleukin-17A (IL-17A), Cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated serine esterase 8 (CTLA-8)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 716004 | 25 µg | $253.00 | |||
This product is not available for shipping outside of the United States.
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8, It also shares 58% homology with an open reading frame of the T-lymphotropic Herpesvirus Samirii virus (viral IL-17). IL-17A belongs to a family of cytokines, which has six members; designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4+ T cells (Th17) that develop in response to IL-23, in particular under conditions in which Th1 and Th2 development are suppressed. IL-17A shares the greatest homology (55%) with IL-17F. Both IL-17A and IL-17F are produced by Th17 cells. IL-17A and IL-17F can either exist as IL-17A homodimers and IL-17F homodimers or as IL-17A-IL-17F heterodimers. IL-17 is a key mediator of autoimmune disorders, including rheumatoid arthritis, psoriasis, inflammatory bowel disease, and asthma, and plays a role in host defense.
Product Details
- Source
- Human IL-17A, amino acids Ile20-Ala155 (Accession# NM_002190), was expressed in E. coli.
- Molecular Mass
- The 137 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15.7 kD. The predicted N-terminal amino acid is Met. This protein exists as a disulfide-linked homodimer.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5.0 x 105 units/mg as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts.
- Application
-
Bioassay
Antigen Details
- Structure
- Homodimer
- Distribution
- IL-17A is largely produced by activated memory T cells, CD4+ T helper cells (Th17), neutrophils, CD8+, NK cells, and γδ T cells.
- Function
- IL-17A is a potent regulator of granulopoiesis and neutrophil recruitment under normal and inflammatory conditions. Organ overexpression of IL-17A increases circulating neutrophil numbers and recruitment into the organs by induction of CXCL2, IL-1β, and G-CSF. IL-17A induces CXCL1, CXCL2, CXCL5, and CXCL8 in human epithelial cells. IL-17A also cooperates with TLR ligands, IL-1β, and TNF-α to enhance inflammatory reactions and stimulate production of beta-defensins and other anti-microbial peptides.
- Interaction
- IL-17RA is expressed in epithelial cells, fibroblasts, B and T cells, myelomonocytic cells, and marrow stromal cells. Synovial endothelial cells and chondrocytes from arthritic patients express IL-17R.
- Ligand/Receptor
- IL-17A signals through a heteromeric receptor composed of IL-17RA and IL-17RC.
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Yu J, et al. 2008. Front. Biosci. 13:170.
2. Toy D, et al. 2006. J. Immunol. 177:36.
3. Benghiat FS, et al. 2009. Transplant Rev. 23:11
4. Honorati MC, et al. 2001. Rheumatology 40:522.
5. Rouvier E, et al. 1993. J. Immunol. 150:5445.
6. Liang SC, et al. 2007. J. Immunol. 179:7791.
7. Ouyang W, et al. 2008. Immunity 28:454. - Gene ID
- 3605 View all products for this Gene ID
- UniProt
- View information about IL-17A on UniProt.org