- Regulatory Status
- RUO
- Other Names
- Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated serine esterase 8, CTLA-8, IL-17
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Induction of IL-6 in human dermal fibroblast by IL-17A.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 570502 | 10 µg | $147.00 | |||
| 570504 | 25 µg | $229.00 | |||
| 570506 | 100 µg | $645.00 | |||
IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8, and share 58% homology with an open reading frame of the T-lymphotropic Herpesvirus Samirii virus (viral IL-17) (5). IL-17A belongs to a family of cytokines, which has five members; designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4 T cells (Th17) that develop in response to IL-23, in particular under conditions in which Th1 and Th2 development are suppressed. IL-17A shares the greatest homology (55%) with IL-17F. Both IL-17A and IL-17F are produced by Th17 cells. IL-17A and IL-17F can either exist as IL-17A homodimers and IL-17F homodimers or as IL-17A-IL-17F heterodimers (6). IL-17 is a key mediator of autoimmune disorders, including rheumatoid arthritis, psoriasis, inflammatory bowel disease, and asthma, and plays a role in host defense (7).
Product Details
- Source
- Human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) was expressed in E. coli.
- Molecular Mass
- The 137 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15,666 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 16kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer, at approximately 28kDa by SDS-PAGE.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 10mM NaH2PO4, 300mM NaCl, pH 7.2.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human IL-17A induces IL-6 production in human neonate dermal fibroblasts in a dose-dependent manner. The ED50 for this effect is 0.06 – 0.36 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Cytokine
- Distribution
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IL-17A is largely produced by activated memory T lymphocytes, CD4+ T helper cells (Th17), neutrophils, CD8(+), NK, and gamma-delta T cells (3).
- Function
- IL-17A is a potent regulator of granulopoiesis and neutrophil recruitment under normal and inflammatory conditions. Organ overexpression of IL-17A increases circulating neutrophil numbers and recruitment into the organs by induction of CXCL2, IL-1β, and G-CSF. Il-17A induces CXCL1, CXCL2, CXCL5, and CXCL8 in human epithelial cells. IL-17A also cooperates with TLR ligands, IL-1 beta, and TNF alpha to enhance inflammatory reactions and stimulate production of beta-defensins and other antimicrobial peptides (1, 2).
- Interaction
- IL-17AR is expressed in epithelial cells, fibroblasts, B and T lymphocytes, myelomonocytic cells, marrow stromal cells, synovial endothelial cells and chondrocytes from arthritic patients express IL-17R (4).
- Ligand/Receptor
- IL-17A signals through a heteromeric receptor composed of IL-17RA and IL-17RC.
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Yu J, et al. Front Biosci 13:170-177 2008.
2. Toy D, et al. J. Immunol. 177:36-39 2006.
3. Benghiat FS, et al. Transplant Rev 23:11-18 2009.
4. Honorati MC, et al. Rheumatology 40:522-527 2001.
5. Rouvier E, et al. J. Immunol. 150:5445-5456 1993.
6. Liang SC, et al. J. Immunol. 179:7791-7799 2007.
7. Ouyang W, et al. Immunity 28:454-467 2008. - Gene ID
- 3605 View all products for this Gene ID
- UniProt
- View information about IL-17A on UniProt.org