- Regulatory Status
- RUO
- Other Names
- Interleukin-17E (IL-17E), Interleukin-25 (IL-25)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 711504 | 25 µg | $253.00 | |||
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IL-17E, also known as IL-25, shares the least structural homology with other IL-17 family members and is also functionally distinct from other IL-17 familiy members. IL-17E is primarily involved in type 2 immune response. IL-17E can induce Th2 cytokines, including IL-4, IL-5, IL-9 and IL-13, in multiple tissues. IL-17E can also inhibit pro-inflammatory Th1 and Th17 cytokine response. It has been well established that IL-17E plays an important role in allergy and asthma. Blocking IL-25 has been a therapeutic option. IL-25 is also required for protection against gastrointestinal helminth infections. IL-17E-deficient mice exihibit impaired Th2 immune response. Exclusion of helminth parasites was delayed in IL-17E-deficient mice. Recently, the potential neuroprotective effect of IL-17E is also being explored. IL-17E can regulate the development of autoimmune inflammation mediated by IL-17-prodcuing T cells. IL-17E-deficient mice are highly susceptible to experimential autoimmune encephalomyelitis. Interestingly, in vivo antitumor activity of IL-17E has also been reported.
Product Details
- Source
- Human IL-17E, amino acids Try33-Gly177 (Accession# Q9H293), was expressed in E. coli.
- Molecular Mass
- The 145 amino acid recombinant protein has a predicted molecular mass of approximately 17 kD. Recombinant human IL-17 is a disulfide-linked homodimer (~34 kD). The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- Determined by its ability to induce IL-8 in human PBMCs using a concentration range of 10 - 100 ng.
- Application
-
Bioassay
Antigen Details
- Structure
- Homodimer
- Distribution
- IL-17E can be secreted by many cells in immune system including CD4+ cells and CD8+ T cells, macrophage, dendritic cells, mast cells and eosinophils, type 2 helper T cells (Th2) and mast cells. IL-17E can also be secreted by non-immune cells including epithelial and endothelial cells.
- Function
- IL-17E is a key requirement for the regulation of type 2 immune response and plays an important role in asthma, allergy, gastrointestinal helminth infection, and autoimmune inflammation. In human asthmatic tissue, IL-25 expression is elevated compare to healthy control.
- Interaction
- CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells.
- Ligand/Receptor
- Heterodimer of IL-17RA/IL-17RB.
- Biology Area
- Cell Biology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Rickel EA. et al. 2008. J. Immunol. 181:4299.
2. Barlow JL, et al. 2009. Biofactors. 35:178.
3. Benatar T, et al. 2010. Cancer. Immunol. Immunother. 59:805.
4. Saene SA, et al. 2010. Nature. 464:1362.
5. Kang A, et al. 2012. Immunity. 36:821. - Gene ID
- 64806 View all products for this Gene ID
- UniProt
- View information about IL-17E on UniProt.org