- Regulatory Status
- RUO
- Other Names
- Interleukin-18 receptor 1, IL-18R-1, IL-18R1, CD218 antigen-like family member A, CD218a, CDw218a, IL1 receptor-releated protein, IL-1Rrp, IL-18R-alpha, IL-18Ralpha, Interleukin-18 receptor alpha, IL-18Ra, IL-1R5
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Recombinant human IL-18Rα-Fc chimera inhibits the induction of IFN-γ by human IL-18 in human KG-1 cells. The ED50 for this effect is 1 - 5 µg/mL. -
Stability Testing for Recombinant Human IL-18Rα-Fc chimera. Recombinant human IL-18Rα-Fc chimera was aliquoted in PBS, pH7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4⁰C (Control). The samples were tested for their ability to inhibit the induction of IFN-γ by human IL-18 in human KG-1 cells. The ED50 for this effect is 1 - 5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 790304 | 25 µg | $147.00 | |||
| 790306 | 100 µg | $335.00 | |||
| 790308 | 500 µg | $996.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-18 is essential for both innate and adaptive immunity. The IL-18 receptor is composed of an α and a β subunit that form a high affinity heterodimer for IL-18. IL-18R1, also known as IL-18Rα chain and CD218a, is a type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of IL-18R1 and IL-18RAcP (IL-18Rβ) on lymphocytes are upregulated upon IL-12 stimulation. IL-23 and IL-18 synergistically enhance IL-18R1 expression on NK-like T cells. Upon IL-18 engagement, the intracellular TIR domain of IL-18R1 and IL-18RAcP recruits adaptor proteins, MyD88, and in turn recruits IRAK and TRAF6, activating NF-κB and MAPK pathways, which upregulates production of various inflammatory cytokines. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. IL-37 also binds to IL-18R1, but recruits IL-1R8 (SIGIRR) to activate anti-inflammatory responses.
Product Details
- Source
- Human IL-18Rα, amino acid Glu20-Arg329 (Accession #Q13478.1), with a linker (SR) and a C-terminal human IgG (Pro100-Lys330) and a 6His tag, was expressed in CHO cells.
- Molecular Mass
- The 549 amino acid recombinant protein has a predicted molecular mass of approximately 62.7 kD. The DTT-reduced and non-reduced protein migrates at approximately 95 kD and 190 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Glu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 is 1 - 5 μg/mL, as determined by a dose-dependent inhibition of human IL-18-induced IFN-γ secretion by KG-1 cells in the presence of 10 ng/mL of human IL-18 (Cat. No. 592102) and 30 μg/mL of human IL-18RAcP (IL-18Rβ).
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines.
- Function
- IL-18Rα forms a receptor complex with IL-18Rβ and mediates IL-18 function.
- Interaction
- MyD88
- Ligand/Receptor
- IL-18
- Bioactivity
- Human IL-18Rα inhibits the induction of IFN-γ by human IL-18 in human KG-1 cells.
- Antigen References
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1. Torigoe K, et al. 1997. J. Biol. Chem. 272: 25737.
2. Born TL, et al. 1998. J. Biol. Chem. 273: 29445.
3. Airoldi I, et al. 2000. J. Immunol. 165: 6880.
4. van de Wetering D, et al. 2008. Int. Immunol. 21: 145.
5. Adachi O, et al. 1998. Immunity. 9: 143.
6. Tsutsumi N, et al. 2014. Nat. Comm. 5: 5340. - Gene ID
- 8809 View all products for this Gene ID
- UniProt
- View information about IL-18Ralpha on UniProt.org