- Regulatory Status
- RUO
- Other Names
- IL-18 receptor accessory protein, IL-18RAP, Accessory protein-like, AcPL, CDw218b, CD218b, IL-1R accessory protein-like, IL-1RAcPL, IL-1R7, IL-18R-beta, IL-18Rbeta, IL-18Rb
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Recombinant human IL-18Rβ-Fc chimera inhibits the induction of IFN-γ by human IL-18 in human KG-1 cells. The ED50 for this effect is 1 – 5 µg/mL. -
Stability Testing for Recombinant Human IL-18Rβ-Fc Chimera. Recombinant human IL-18Rβ-Fc chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4⁰C (Control). The samples were tested for their ability to inhibit the induction of IFN-γ by human IL-18 in human KG-1 cells. The ED50 for this effect is 1 – 5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 790404 | 25 µg | $147.00 | |||
| 790406 | 100 µg | $335.00 | |||
| 790408 | 500 µg | $996.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-18 is essential for both innate and adaptive immunity. IL-18 receptor is composed of an α and a β subunit that form a high affinity heterodimer for IL-18. IL-18Rβ chain, also known as IL-18RAcP and CD218b, is a type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of IL-18Rα and IL-18Rβ on lymphocytes are upregulated upon IL-12 stimulation. Upon IL-18 engagement, the intracellular TIR domain of IL- IL-18Rα and IL-18Rβ recruits adaptor proteins, MyD88, and in turn recruits IRAK and TRAF6, activating the NF-κB and MAPK pathways, which upregulate the production of various inflammatory cytokines. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. Antibodies against IL-18Rβ inhibit IL-18-induced responses. Several splice isoforms of IL-18Rβ were identified thus, a known polymorphism on the IL-18Rβ gene leads to reduced expression of IL-18Rβ and reduced pattern-recognition receptor (PRR)-induced cytokine secretion in monocyte-derived macrophages.
Product Details
- Source
- Human IL-18Rβ, amino acid Phe20-Arg356 (Accession #O95256.1), with a linker (SSLGS) and a C-terminal human IgG (Pro100-Lys330), was expressed in CHO cells.
- Molecular Mass
- The 573 amino acid recombinant protein has a predicted molecular mass of approximately 64.82 kD. The DTT-reduced and non-reduced protein migrates at approximately 90 kD and 180 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Phe.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution in PBS
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 is 1 - 5 μg/mL, as determined by a dose-dependent inhibition of human IL-18-induced IFN-γ secretion by KG-1 cells in the presence of 10 ng/mL human IL-18 (Cat. No. 592102) and 30 μg/mL human IL-18Rα.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines; plasma membrane
- Function
- IL-18Rα forms a receptor complex with IL-18Rβ and mediates IL-18 function.
- Interaction
- MyD88
- Ligand/Receptor
- IL-18
- Bioactivity
- Human IL-18Rβ inhibits the induction of IFN-γ by human IL-18 in human KG-1 cells.
- Cell Type
- B cells, Endothelial cells, Leukocytes, Lymphocytes, Neutrophils, NK cells, T cells, Th1
- Biology Area
- Adaptive Immunity, Immunology, Innate Immunity
- Molecular Family
- Fc Receptors, Soluble Receptors
- Antigen References
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1. Born TL., et al. 1998. J Biol Chem. 273: 29445.
2. Airoldi I., et al. 2000. J Immunol. 165: 6880.
3. Debets R., et al. 2000. J Immunol. 165: 4950.
4. Adachi O., et al. 1998. Immunity. 9: 143.
5. Tsutsumi N.. et al. 2014. Nat Comm. 5: 5340.
6. Hedl M., et al. 2014. J Immunol. 192: 5924.
7. Fiszer D., et al. 2007. Cytokine 39: 178.
8. Kato Z., et al. 2003. Nat Struc Biol. 10: 966. - Gene ID
- 8807 View all products for this Gene ID
- UniProt
- View information about IL-18Rbeta on UniProt.org