- Regulatory Status
- RUO
- Other Names
- Catabolin, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta.
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Recombinant human IL-1β stimulates proliferation of D10.G4.1 cells in a dose-dependent manner. BioLegend’s protein was compared side-by-side to a competitor’s equivalent product. The ED50 for this effect is 2.5 – 12.5 pg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 579402 | 10 µg | $206.00 | |||
| 579404 | 25 µg | $347.00 | |||
| 579406 | 100 µg | $1113.00 | |||
| 579408 | 500 µg | $2461.00 | |||
IL-1β in humans and mice does not encode a typical signal peptide and, as a result, newly synthesized pro-IL-1β accumulates within the cytoplasm of activated monocytes and macrophages (1). Conversion of the inactive pro-IL-1β to its mature form requires the proteolytic action of IL-1β-converting enzyme (ICE), also termed caspase-1 (2). Secretion of mature IL-1β from LPS-activated monocytes/macrophages is not a constitutive process. These cells must encounter a secondary stimulus that specifically activates the posttranslational processing events (2). Moreover, owing to its pro-inflammatory nature, IL-1β is regarded as a tumor-promoting cytokine. In fact, enhanced tumor metastasis and angiogenesis has been observed under the influence of IL-1β (3). IL-1β is able to facilitate tumor progression in murine models of lung cancer. In addition, upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor (4).
Product Details
- Source
- Human IL-1β, amino acids Ala117-Ser269 (Accession # NM_000576) was expressed in E. coli.
- Molecular Mass
- The 153 amino acid recombinant protein has a predicted molecular mass of 17,376 Da. The DTT-reduced and the non-reduced protein migrate at approximately 18kDa by SDS-PAGE. The N-terminal amino acid is Alanine.
- Purity
- Purity is > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
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Human IL-1β induces the proliferation of D10.G4.1 mouse helper lymphocytes in a dose-dependent manner. The ED50 = 2.5 - 12.5 pg/mL.
The specific activity of recombinant human IL-1β is approximately 5.63 x 104 IU/µg when compared against the 1st WHO International Standard for Human IL-1β (NIBSC code: 86/680) as determined by the dose dependent stimulation of D10.G4.1 cell proliferation.
For more information on specific activity, please visit the Recombinant Protein Unit Conversions page. - Application
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Bioassay
- Recommended Usage
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Use when high specific biological activity is required.
- Application Notes
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This IL-1β protein is biologically active, and can be used for in vitro assays
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com. - Product Citations
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Antigen Details
- Structure
- Cytokine
- Function
- Interleukin (IL)-1β is a cytokine with a key role in the pathophysiology of local and systemic inflammation. IL-1β induces cytokine, chemokine, proinflammatory molecule secretion, and adhesion molecule expression in diverse cells.
- Ligand/Receptor
- Type I and II IL-1 receptors (IL-1RI and IL-1RII)
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Stevenson FT, et al. J Cell Physiol 152:223-231 1992.
2. Shi J, et al. J. Immunol. 179:1245-1253 2007.
3. Dinarello CA Cancer Metastasis Rev 25:307-313 2006.
4. Boost KM, et al. BMC Cancer 8:265 2008. - Gene ID
- 3553 View all products for this Gene ID
- UniProt
- View information about IL-1beta on UniProt.org