- Regulatory Status
- RUO
- Other Names
- IL-1RA, ICIL-1RA, IRAP, IL-1RN
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Human IL-1RA inhibits the D10.G4.1 cell proliferation induced by IL-1α.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 714406 | 100 µg | $253.00 | |||
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Interleukin-1 receptor antagonist (IL-1RA or IL-RN) is a member of IL-1 family cytokine and it is a naturally occurring inflammatory inhibitor protein. It competes with IL-1α and IL-1β for binding to type I and type II receptors. It can modulate a variety of interleukin 1 related immune and inflammatory responses. IL-1RA has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo model. Recombinant IL-1RA (anakinra) has been used as to treat rheumatoid arthritis. A polymorphism of IL-1RN gene is reported to be associated with increased risk of osteoporotic fractures, gastric cancer, bladder cancer, and other cancer types.
Product Details
- Source
- Human IL-1R antagonist (IL-1RA), amino acids (Arg26-Glu177) (P18510), was expressed in E. coli.
- Molecular Mass
- The 153 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 17.2 kD. The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 7 - 35 ng/ml, corresponding to a specific activity of 0.2 - 1.4 x 105 units/mg, as determined by inhibition of D10.G4.1 cell proliferation induced by human IL-1α.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and mouse.
Antigen Details
- Structure
- Cytokine inhibitor
- Distribution
- Corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts.
- Function
- Inhibits the activity of IL-1β and IL-1α by binding to receptor IL1R1 and blocking downstream signaling.
- Ligand/Receptor
- Type I and II IL-1 receptors (IL-1RI and IL-1RII) for human.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Stem Cells, Cell Biology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Zhang Y, et al. 2012. PLoS One 7:e46017.
2. Schrijver HM, et al. 2003. Mediators Inflamm. 12:89.
3. Konwar R, et al. 2009. Oncol Res. 17:362.
4. Muzio M, et al. 1995. J. Exp. Med. 182:623. - Gene ID
- 3557 View all products for this Gene ID
- UniProt
- View information about IL-1RA on UniProt.org