- Regulatory Status
- RUO
- Other Names
- T-cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
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Recombinant human IL-2 induces the proliferation of HT-2 mouse cells in a dose-dependent manner. The ED50 for this effect is 0.3 – 1.5 ng/mL. -
Stability Testing for Recombinant Human IL-2. Recombinant human IL-2 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to induce the proliferation of HT-2 mouse cells in a dose-dependent manner. The ED50 for this effect is 0.3 – 1.5 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 566904 | 25 µg | $180.00 | |||
| 566906 | 100 µg | $395.00 | |||
IL-2 was discovered through its function as a T cell growth factor (TCGF). It plays a pivotal role in immune responses against pathogenic infection. Recognition and binding of foreign antigens by the TCRs stimulate both the secretion of IL-2 and the expression of IL-2Rs on the T cell surface. Subsequently, the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT signaling pathways, and ultimately stimulates the growth, differentiation, and survival of the Ag-selected cytotoxic T cells. Human IL-2 acts on murine and human T cells, and its receptors are shared by other cytokines. IL-2Rα is an IL-2–specific receptor, IL-2Rβ is shared with IL-15 and the γ chain that is a common receptor shared by many cytokines including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
Product Details
- Source
- Human IL-2, amino acid sequence (Ala21-Thr153) (Accession: # P60568), was expressed in 293E cells. The amino-terminus contains 8His tag.
- Molecular Mass
- The 151 amino acid recombinant protein has a predicted molecular mass of approximately 17.4 kD. The DTT-reduced and non-reduced proteins migrate at approximately 15 kD - 18 kD by SDS-PAGE. The predicted N-terminal amino acid is His.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human IL-2 induces the proliferation of HT-2 cells in a dose dependent manner. The ED50 for this effect is 0.3 – 1.5 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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T cells (CD4+ and CD8+), gamma δ T cells, B cells, dendritic cells, and eosinophils. Mainly activated T cells
- Function
- Differentiation of Th1 and Th2 cells, inhibition of Th17 and Tfh cell differentiation, regulation of Treg differentiation, T cell proliferation, B cell proliferation, promotes CD8 and NK cell cytolytic activity
- Interaction
- CD4 T cells, T reg cells, CD8 effector cells, NK cells, B cells
- Ligand/Receptor
- Trimeric receptor: IL-2Rα (CD25), IL-2Rβ (CD122), and γc (CD132) and dimeric receptor: IL-2Rβ (CD122) and γc (CD132)
- Bioactivity
- Recombinant human IL-2 induces the proliferation of mouse HT-2 cells in a dose dependent manner.
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Siegel JP, et al. 1987. Science. 238:75-8.
- Cote-Sierra J, et al. 2004. Proc Natl Acad Sci USA. 101:3880-5.
- Laurence A, et al. 2007. Immunity. 26:371-81
- Sakaguchi S, et al. 2008. Cell. 133:775-87.
- Liao W, et al. 2011. Nat Immunol. 12:551-9.
- Ballesteros-Tato A, et al. 2012. Immunity. 36:847-56.
- Liao W, et al. 2013. Immunity. 38:13-25.
- Read KA, et al. 2016. Exp Hematol. 44:799-808.
- Gene ID
- 3558 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org