- Regulatory Status
- RUO
- Other Names
- T-cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
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Recombinant human IL-2 induces the proliferation of mouse HT-2 cells in a dose-dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL. -
Stability Testing for Recombinant Human IL-2. Recombinant human IL-2 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4°C (Control). The samples were tested for their ability to induce the proliferation of mouse HT-2 cells in a dose-dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 791902 | 10 µg | $83.00 | |||
| 791904 | 25 µg | $112.00 | |||
| 791906 | 100 µg | $223.00 | |||
| 791908 | 500 µg | $528.00 | |||
IL-2 was discovered through its function as a T cell growth factor (TCGF). It plays a pivotal role in immune responses against pathogenic infection. Recognition and binding of foreign antigens by the TCRs stimulate both the secretion of IL-2 and the expression of IL-2Rs on the T cell surface. Subsequently, the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT signaling pathways, and ultimately stimulates the growth, differentiation, and survival of the Ag-selected cytotoxic T cells. Human IL-2 acts on murine and human T cells, and its receptors are shared by other cytokines. IL-2Rα is an IL-2–specific receptor, IL-2Rβ is shared with IL-15 and the γ chain that is a common receptor shared by many cytokines including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
Product Details
- Source
- Human IL-2, amino acid (Ala21-Thr153) (Accession: # P60568) was expressed in E. coli.
- Molecular Mass
- The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 15 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in 0.1% TFA, 10% Acetonitrile
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human IL-2 induces the proliferation of mouse HT-2 cells in a dose- dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Monomer
- Distribution
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T cells (CD4+ and CD8+), gamma δ T cells, B cells, dendritic cells, and eosinophils. Mainly activated T cells.
- Function
- Differentiation of Th1 and Th2 cells, inhibition of Th17 and Tfh cell differentiation, regulation of Treg differentiation, T cell proliferation, B cell proliferation, promotes CD8 and NK cell cytolytic activity.
- Interaction
- CD4 T cells, T reg cells, CD8 effector cells, NK cells, B cells
- Ligand/Receptor
- Trimeric receptor: IL-2Rα (CD25), IL-2Rβ (CD122), and γc (CD132) and dimeric receptor:IL-2Rβ (CD122) and γc (CD132)
- Bioactivity
- Recombinant human IL-2 induces the proliferation of mouse HT-2 cells.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
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- Siegel JP, et al. 1987. Science. 238:75-8.
- Cote-Sierra J, et al. 2004. Proc Natl Acad Sci U S A. 101:3880-5.
- Laurence A, et al. 2007. Immunity. 26:371-81.
- Sakaguchi S, et al. 2008. Cell. 133:775-87.
- Liao W, et al. 2011. Nat Immunol. 12:551-9.
- Ballesteros-Tato A, et al. 2012. Immunity. 36:847-56.
- Liao W, et al. 2013. Immunity. 38:13-25.
- Read KA, et al. 2016. Exp Hematol. 44:799-808.
- Gene ID
- 3558 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org