- Regulatory Status
- RUO
- Other Names
- IL22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 716202 | 10 µg | $253.00 | |||
This product is not available for shipping outside of the United States.
IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79% amino acid identity with mouse IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4+ effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.
Product Details
- Source
- Human IL-22, amino acids Ala34-Ile179 (Accession # NM_020525) with N terminus Met, was expressed in E. coli.
- Molecular Mass
- The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16.9 kD. The predicted N-terminal amino acid is Met.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.7 - 1.0 ng/ml, corresponding to a specific activity of 1.0 - 1.4 x 106 units/mg as determined by the dose-dependent stimulation of human Colo205 cells in production of IL-10.
- Application
-
Bioassay
Antigen Details
- Structure
- Cytokine
- Distribution
- Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells.
- Function
- Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis.
- Interaction
- Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma.
- Ligand/Receptor
- IL-22R1 and IL-10R2 receptor chains.
- Bioactivity
- IL-22 bioactivity was measured by its ability to induce IL-10 in human Colo205 cells.
- Biology Area
- Cell Biology, Stem Cells, Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Nagalakshmi ML, et al. 2004. Intern. Immunopharmacol. 4:679.
2. Kebir H, et al. 2007. Nat. Med. 13:1173.
3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807.
4. Pene J, et al. 2008. J. Immunol. 180:7423.
5. Dumotier L, et al. 2000. J. Immun. 164:1814.
6. Xie MH, et al. 2000. J. Biol. Chem. 765:31335.
7. Jones BC, et al. 2008. Structure.
8. Kotenko SV, et al. 2001. J. Immunol. 166:7096
9. Chang C, et al. 2006. Cell Res 16:902. - Gene ID
- 50616 View all products for this Gene ID
- UniProt
- View information about IL-22 on UniProt.org