- Regulatory Status
- RUO
- Other Names
- IL22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 571302 | 10 µg | $229.00 | |||
| 571304 | 25 µg | $464.00 | |||
| 571306 | 100 µg | $1055.00 | |||
| 571308 | 500 µg | $2929.00 | |||
IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells.IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79% amino acid identity with mouse IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4 (+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.
Product Details
- Source
- Human IL-22, amino acids Ala34-Ile179 (Accession # NM_020525) was expressed in E. coli.
- Molecular Mass
- The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,880 Da. The DTT-reduced protein migrates at approximately 15kDa and the non-reduced protein migrates at approximately 14kDa by SDS-PAGE.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH7.2.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 is 0.062-0.177 ng/ml, corresponding to a specific activity of 1.61-0.56 X107 units/mg, as determined by a dose dependent stimulation of human Colo205 cells in production of IL-10.
- Application
-
Bioassay
- Recommended Usage
-
Use when high specific biological activity is required.
- Application Notes
-
Ligand Blocking: This IL-22 protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining.
Bioassay: This IL-22 protein is biologically active, and can be used for in vitro assays.
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com. -
Application References
(PubMed link indicates BioLegend citation) -
- Dhiman R, et al. 2009. J. Immunol. 183:6639. Pubmed
- Product Citations
-
Antigen Details
- Structure
- Cytokine
- Distribution
- thymic lymphomas, T cells, activated T cells, macrophages, and mast cells
- Function
- Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
- Interaction
- Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
- Ligand/Receptor
- IL-22R1 and IL-10R2 receptor chains
- Bioactivity
- IL-22 bioactivity was measured by its ability to induce IL-10 in human Colo205 cells
- Biology Area
- Stem Cells, Immunology, Cell Biology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Nagalakshmi ML, et al. Intern Immunopharmacol 4:679-691 2004.
2. Kebir H, et al. Nat. Med. 13:1173-1175 2007.
3. Gu Y, et al. Eur. J. Immunol. 38:1807-1813 2008.
4. Pene J, et al. J. Immunol. 180:7423-7430 2008.
5. Dumotier L, et al. J immune 164:1814-1819 2000.
6. Xie MH, et al. J. Biol. Chem. 765:31335-31339 2000.
7. Jones BC, et al. Structure July 2 2008.
8. Kotenko SV, et al. J. Immunol. 166:7096-71033 2001.
9. Chang C, et al. Cell Res 16:902-907 2006. - Gene ID
- 50616 View all products for this Gene ID
- UniProt
- View information about IL-22 on UniProt.org