- Regulatory Status
- RUO
- Other Names
- Interleukin-2 receptor subunit beta, IL-2RB, IL-2 receptor subunit beta, IL-2R subunit beta, high affinity IL-2 receptor subunit beta, IL-15RB, Interleukin-15 receptor subunit beta, CD122
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IL-2Rβ inhibits MO7e cell proliferation induced by human IL-15 at 10 ng/ml. -
Stability testing for human IL-2Rβ. Human IL-2Rβ was aliquoted in PBS at 0.2 mg/ml; one aliquot was kept at 4°C (control) and another was freeze-thawed four times (4 x freeze-thaws). After this procedure, the samples were tested by its property to inhibit the proliferation of MO7e cells induced by human IL-15 (10 ng/ml, Cat. No. 570302).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 773402 | 10 µg | $159.00 | |||
| 773404 | 25 µg | $218.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-2Rβ, also known as IL-15RB, is a type I transmembrane protein. IL-2Rβ is one subunit of IL-2 receptor, which is constituted by α (CD25), β (CD122), and common γ (CD132) chains. The IL-2Rβ associates with either the common γ subunit alone or with the γc subunit and the IL-2Rα subunit to generate intermediate or high affinity IL-2 receptor complexes, respectively. IL-2Rβ and γc are also shared by the IL-15 receptor. Upon IL-2 ligation, the IL-2 receptor complexes induce the downstream signaling pathways, including JAK/STAT, PI3K and Ras/MAPK pathways. Though the IL-2 receptor has no intrinsic kinase activity, IL-2Rβ and γc were found to be associated with tyrosine kinases, JAK1 and JAK3, respectively, and recruit adaptor proteins. Phosphorylated IL-2Rβ is necessary for transducing the signaling. IL-2Rβ is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages, but its expression levels can be upregulated by activation. It has been reported that IL-2Rβ is cleaved and released from the cell surface though the function of soluble form in vivo is not clear yet. High level of soluble IL-2R in serum has been observed in autoimmune diseases like systemic lupus erythematosus and rheumatoid arthritis. In IL-2Rβ gene deficient mice, T cells are activated and it is observed exhaustive B cell differentiation, indicating that IL-2Rβ is involved in the maintenance of T cell homeostasis. IL-2Rβ is associated with autoimmune diseases. It has been reported that IL-2Rβ expression is reduced on CD4+ T cell in ANCA (antineutrophil-cytoplasmic-antibodies)-associated vasculitis. In addition, polymorphisms in IL-2Rβ gene were identified to associated with inflammatory bowel disease based on genome-wide studies.
Product Details
- Source
- Human IL-2Rβ, amino acid (Ala27-Asp239) (Accession # P14784.1), was expressed in CHO cells.
- Molecular Mass
- The 213 amino acid recombinant protein has a predicted molecular mass of approximately 24.6 kD. The DTT-reduced and non-reduced protein migrates at approximately 40 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0. 1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 = 0.3 – 1.8 µg/mL as determined by its ability to inhibit MO7e cell proliferation induced by human IL-15 (10 ng/ml, Cat. No. 570302). Deep Blue Cell Viability™ Kit (Cat. No. 424702) was used to measure cell proliferation.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Cytokine receptor
- Distribution
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NK cells, T cells, B cells, monocytes, and macrophages.
- Function
- IL-2Rβ is one subunit of IL-2 receptor and involved in the maintenance of T cell homeostasis.
- Interaction
- SHB adaptor protein, JAK1
- Ligand/Receptor
- IL-2, IL-15
- Bioactivity
- Inhibits the proliferation of MO7e cells.
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
-
- Miyazaki T, et al. 1994. Science. 266: 1045.
- Lindholm CK, et al. 2002. Biochem Biophys Res Commun. 296: 929.
- Gaffen SL. 2001. Cytokine. 14: 63.
- Honda M, et al. 1990. J Immunol. 145: 4131.
- Montes de Oca P, et al. 2010. J Biol Chem. 285: 22050.
- Suzuki H, et al. 1995. Science. 268: 1472.
- Phillips JH, et al. 1989. J Exp Med. 170: 291.
- Wilde B, et al. 2014. Dis Markers. 2014: 249846.
- Ruiz-Medina BE, et al. 2015. J Biol Chem. 290: 20972.
- Bouzid D, et al. 2013. Genet Test Mol Biomarkers. 17: 833.
- Gene ID
- 3560 View all products for this Gene ID
- UniProt
- View information about IL-2RB on UniProt.org