- Regulatory Status
- RUO
- Other Names
- Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
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Recombinant Human IL-3 induces the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner. The ED50 for this effect is 0.25 – 1.25 ng/mL. -
Stability Testing for Recombinant Human IL-3. Recombinant Human IL-3 was aliquoted in PBS, pH 6.5 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to induce the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner. The ED50 for this effect is 0.25 – 1.25 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 564304 | 25 µg | $330.00 | |||
| 564306 | 100 µg | $940.00 | |||
Interleukin-3 (lL-3) is a hemopoietic growth factor involved in the survival, proliferation, and differentiation of multipotent hemopoietic cells. In addition to granulocyte-macrophage colony-stimulating factor (GMCSF) and IL-5, IL-3 is the most potent growth factor for basophils. On mature basophils, these three cytokines act through specific receptors mediating adhesion, migration, and releasability. Mast cells expresses high amounts of IL-3. In the absence of antigen, rapid release of large amount of IL-3 in an autocrine dependent manner is responsible for mast cell survival. IL-3 plays a vital role in stimulating basophils and mast cell responses to parasite infections. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. IL-3 is expressed in the major embryonic vessels and regulates the survival and proliferation of hematopoietic stem cells in the early stages of embryonic development.
Product Details
- Source
- Recombinant human IL-3, amino acid sequence containing Ala20-Phe152 (Accession No. NP-000579), was expressed in E.coli. The carboxy-terminus contains a linker-6His tag.
- Molecular Mass
- The 146 amino acid recombinant protein has a predicted molecular mass of approximately 16.24 kD. The DTT-reduced and non-reduced proteins migrate at approximately 16 kD - 18 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 6.5
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant Human IL-3 induces the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.25 – 1.25 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, NK cells, and endothelial cells
- Function
- IL-3 is a pleiotropic factor which promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections.
- Interaction
- Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
- Ligand/Receptor
- Heterodimeric receptor formed by IL-3Rα (CD123) and a common β subunit (βc) that is shared with the IL-5R and GM-CSFR
- Bioactivity
- Measured by its ability to induce the proliferation of TF-1 human erythroleukemic cells.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Kitamura T, et al. 1989. J Cell Physiol. 140:323-34.
- Kohno M, et al. 2005. Blood. 105:2059-65
- Murphy JM and Young IG. 2006. Vitam Horm. 74:1-30.
- Kleemann R, et al. 2008. Cardiovasc Res. 79:360-76.
- Valent P and Dahinden CA. 2010. Curr Opin Hematol. 17:60-6.
- Mirza S, et al. 2010. J Biol Chem. 285:2237.
- Bénard A, et al. 2021. Nat Commun. 12:1112.
- Gene ID
- 3562 View all products for this Gene ID
- UniProt
- View information about IL-3 on UniProt.org