- Regulatory Status
- RUO
- Other Names
- IL-1F11, Nuclear factor from high endothelial venules (NF-HEV)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 716302 | 10 µg | $253.00 | |||
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IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1β. IL-33, IL-1β, and IL-18 are synthesized as biologically inactive precursors and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and, subsequently, activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1β. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.
Product Details
- Source
- Human IL-33, amino acids Ser112-Thr270 (Accession# NP_ 118656), was expressed in E. coli.
- Molecular Mass
- The 159 amino acid recombinant protein has a predicted molecular mass of 16.9 kD. The predicted N-terminal amino acid is Ser.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2.0 x 107 units/mg as determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and mouse.
Antigen Details
- Structure
- Cytokine
- Distribution
- IL-33 mRNA is expressed in a broad range of human tissues, such as heart, small intestine, brain, ovary, testis, pancreas, and colon. Higher IL-33 expression was detected in human keratinocyte, fibroblast, myocyte, and alveolar cells.
- Function
- IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-α and IL-1β are activators of IL-33 transcription in fibroblasts and keratinocytes.
- Interaction
- Th2 cells
- Ligand/Receptor
- IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein).
- Bioactivity
- Human IL-33 is able to induce proliferation of mouse D10S cells.
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Schmitz J, et al. 2005. Immunity 23:479.
2. Barksby HE, et al. 2007. Clin. Exp. Immunol. 149:217.
3. Arend WP, et al. 2008. Immunol. Rev. 223:20.
4. Suzukawa M, et al. 2008. J. Immunol. 181:5981.
5. Moussion C, et al. 2008. PLoS One 3:e3331.
6. Mildner M, et al. 2010. Cardiovasc. Res. 87:769. - Gene ID
- 90865 View all products for this Gene ID
- UniProt
- View information about IL-33 on UniProt.org