- Regulatory Status
- RUO
- Other Names
- Interleukin-36 gamma (IL-36γ), IL-1 related protein 2 (IL-1RP2), Interleukin 1 family member 9 (IL-1F9), IL-1 epsilon (IL-1ε), IL-1H1.
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Human IL-36γ induces IL-8 production in the human epidermoid carcinoma cell line A431. -
Recombinant human IL-36γ induces IL-8 production in the human epidermoid carcinoma cell line A431 in a dose dependent manner. BioLegend’s protein was compared side-by-side to the leading competitor’s equivalent product. -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 767402 | 10 µg | $218.00 | |||
| 767404 | 25 µg | $364.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
The IL-1 family is a group of cytokines comprised of 11 members, including the recently renamed IL-36 cytokines, IL-36α, β and γ (previously known as IL-1F6, IL-1F8, and IL-1F9). IL-36γ signals through the IL-1 receptor family members IL-1Rrp2 and co-recptor IL-1RAcP which in turn can activate downstream MAP kinases and NF-kB signaling pathway. The activity is antagonized by IL-36Ra/ IL-1F5. IL-36γ is synthesized without signal peptide and the secretion pathway is unclear. Though IL-36γ do not contain caspase-1 cleavage site, It has been shown that, the N-terminal truncated form of IL-36γ can significantly increase its biological activity. The possible processing enzymes are currently unknown. IL-36γ plays a significant role in immune response. Low level of IL-36 receptor IL-1Rrp can be detected on bone marrow-derived dendritic cells and CD4+ T cells. IL-36γ treatment can upgulate CD80, CD86 and MHC Class II, and proinflammatory cytokine producton in dendritic cells. IL-36γ has also been implicated in airway hyperesponsiveness. Intranasal administration of recombinant IL-36γ in mice leads to epithelial cell hypertrophy, cellular infiltration, and mucus production. IL-36γ expression in the lungs of mice can be upregulated by allergic inflammation. In the skin, IL-36γ is primarily expressed by keratinocytes and its expression can be upregulated by TNF-α, IFN-γ, toll-like receptor ligands, and some pathological conditions. IL-36γ is highly induced in psoriasis plaques and can be used as a biomarker.
Product Details
- Source
- Human IL-36γ, amino acids Ser18-Asp169 (Accession #: BC098337.1) was expressed in E. coli.
- Molecular Mass
- The 152 amino acid recombinant protein has a predicted molecular mass of approximately 17 kD. The reduced and non-reduced proteins migrate at approximately 17 kD by SDS-PAGE. The predicted N-terminal amino acid is Ser.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 20 mM MOPS, 150 mM NaCl, 10 mM TCEP, pH 7.5, 10% glycerol.
- Endotoxin Level
- Less than 0.1 EU/µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- The ED50 is 2-10 ng/ml, as determined by a dose-dependent stimulation of IL-8 secretion in A431 cells. IL-8 was measered by ELISA.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Cytokine
- Function
- IL-36γ is a proinflammatory cytokine. It is upregulated by inflammatory cytokines, toll-like receptor ligands, and some pathological conditions in skin. Truncation of IL-36α, IL-36β, and IL-36γ enhances their activity.
- Interaction
- Dendritic cell, macrophages, lung fibroblasts.
- Ligand/Receptor
- Receptor IL-1Rrp2 and co-receptor IL-1RAcP.
- Bioactivity
- Induces IL-8 secretion in human epidermoid carcinoma cell line A431.
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Blumberg H, et al. 2007. J. Exp. Med. 204:2603.
2. Vigne S, et al. 2011. Blood. 118: 5813.
3. Ramadas RA, et al. 2006. Immunogenetics. 58:851.
4. Carrier Y, et al. 2011. J. Invest. Dermatol. 131:2428.
5. Debets R, et al. 2001. J. Immunol. 167:1440.
6. Towne J, et al. 2004. J. Biol. Chem. 279:13677.
7. Towne J, et al. 2011. J. Biol. Chem. 286:42594. - Gene ID
- 56300 View all products for this Gene ID
- UniProt
- View information about IL-36gamma on UniProt.org