- Regulatory Status
- RUO
- Other Names
- B cell growth factor 1 (BCGF-1), B-cell stimulatory factor 1 (BSF-1), interleukin-4, lymphocyte stimulatory factor 1, MGC79402
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Human IL-4 induces proliferation of TF‑1 human erythroleukemic cells. -
Recombinant human IL-4 induces the proliferation of TF-1 human erythroleukemic cells in a dose dependent manner. BioLegend’s protein was compared side-by-side to a competitor’s equivalent product.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 574002 | 10 µg | $147.00 | |||
| 574004 | 25 µg | $253.00 | |||
| 574006 | 100 µg | $762.00 | |||
| 574008 | 500 µg | $2109.00 | |||
IL-4 is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with allergy and asthma. The Type I receptor comprises IL-4Rα and the common gamma-chain (γc), which is also shared by the cytokines IL-2, -7, -9, -15 and -21 and is present in hematopoietic cells. IL-4 can use the type II complex, comprising IL-4Rα and IL-13Rα1, which is present in non-hematopoietic cells. This second receptor complex is a functional receptor for IL-13, which shares approximately 25% homology with IL-4. The type I receptor complex can be formed only by IL-4 and is active in Th2 development. In contrast, the type II receptor complex formed by either IL-4 or IL-13 is more active during airway hypersensitivity and mucus secretion and is not found in T cells.
Product Details
- Source
- Human IL-4, amino acids His25-Ser153 (Accession# NM_000589) was expressed in E.coli.
- Molecular Mass
- The 130 amino acid recombinant protein has a predicted molecular mass of approximately 15.1 kD. The N-terminal amino acid is Met.
- Purity
- Purity is >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- The protein was 0.22 µm filtered in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.01ng per µg cytokine as determined by the LAL method
- Preparation
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer containing carrier protein such as 1% BSA or HSA or 10% FBS.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
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ED50 = 0.04 - 0.2 ng/mL as determined by the dose-dependent stimulation of TF-1 cell proliferation.
The specific activity of recombinant human IL-4 is approximately 1.02 x 104 IU/µg when compared against the 1st WHO International Standard for Human Interleukin-4 (NIBSC code: 88/656) as determined by the dose-dependent stimulation of TF-1 cell proliferation.
For more information on specific activity, please visit the Recombinant Protein Unit Conversions page. - Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
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Application References
(PubMed link indicates BioLegend citation) -
- Chalubinski M, et al. 2014. Food Chem Toxicol. 69:289. PubMed
- Product Citations
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Antigen Details
- Structure
- Heterodimer
- Distribution
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IL-4 is produced by Th2 cells, naive CD4+ T cells, NKT cells, and basophils.
- Function
- IL-4 has a crucial role in the differentiation of TH2 cells and induction of Th2 associated cytokines. IL-4, through its activation of STAT6, upregulates GATA3 expression and also suppresses TH1 and TH17 cell responses, partly through the upregulation of growth factor independent 1(GFI1), a transcriptional repressor of IFNγ and IL-17 production. IL-4 induces macrophage activation and TSLP production. IL-4 recruits and activates IgE-producing B cells (IgE class switching) and enhances IgE-mediated responses by up-regulating IgE receptors on B lymphocytes, mast cells, and basophils. In addition, IL-4 also induces VCAM-1 on vascular endothelium and thus directs the migration of T lymphocytes, monocytes, basophils, and eosinophils to the inflammation site.
- Interaction
- T cells, B cells, macrophages, epithelial cells, smooth muscle cells, and bronchial fibroblasts.
- Ligand/Receptor
- IL-4 signals through Type I (IL-4Rα, γc) and Type II receptors (IL-4Rα, IL-13Rα1) complexes.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Swain SL, et al. 1990. J. Immunol. 145:3796.
2. Hsieh CS, et al. 1992. P. Natl. Acad. Sci. USA 89:6065.
3. Allison-Lynn A, et al. 2006. J. Immunol. 176:7456.
4. Kato A, et al. 2007. J. Immunol. 179:1080.
5. LaPorte SL, et al. 2008. Cell 132:259.
6. Martinez FO, et al. 2009. Annu. Rev. Immunol. 27:451. - Gene ID
- 3565 View all products for this Gene ID
- UniProt
- View information about IL-4 on UniProt.org