- Regulatory Status
- RUO
- Other Names
- Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 560701 | 2 µg | $147.00 | |||
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IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes.
Product Details
- Source
- E. coli, approximately 26 kD
- Formulation
- Sterile-filtered through a 0.2 micron filter. Lyophilized with no additives.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Preparation
- Centrifuge vial prior to opening. Reconstitute in 100 µl sterile water for a concentration of 20 µg/ml and mix well. This solution can be diluted into other aqueous buffers containing a carrier protein such as 1% BSA or HSA or 10% FBS. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer with carrier protein. After reconstitution, the cytokine can be stored at -20°C to -70°C for up to three months.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Application
-
ELISA, Bioassay
- Recommended Usage
-
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. Please see antibody product data sheets and recommended protocols. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA: This IL-5 protein is useful as a standard for a human IL-5 sandwich ELISA, using unlabeled JES1-39D10 antibody (catalog #500902) for capture and biotinylated JES1-5A10 antibody (catalog #501002) for detection.
Ligand Blocking: This IL-5 protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining.
Bioassay: This IL-5 protein is biologically active, and can be used for in vitro assays. - Product Citations
-
Antigen Details
- Structure
- Cytokine; dimer
- Bioactivity
- Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
- Cell Sources
- Mast cells, T cells, bone marrow stromal cells
- Cell Targets
- Eosinophils
- Receptors
- Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
- Cell Type
- Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Immunology, Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immun. 35:593. - Regulation
- Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
- Gene ID
- 3567 View all products for this Gene ID
- UniProt
- View information about IL-5 on UniProt.org