- Regulatory Status
- RUO
- Other Names
- Interleukin 5, B-cell differentiation factor I, T-cell replacing factor, eosinophil differentiation factor, Colony-Stimulating Factor, Eosinophil
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Recombinant human IL-5 induces proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner with an ED50 range of 0.05 - 0.25 ng/mL. -
Stability Testing for Recombinant Human IL-5. Recombinant human IL-5 was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (control). The samples were tested in a proliferation assay with TF-1 human erythroleukemic cells.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 791302 | 10 µg | $171.00 | |||
| 791304 | 25 µg | $293.00 | |||
IL-5 is a homodimeric glycoprotein that was initially identified by its ability to support the in vitro growth and differentiation of mouse B cells and eosinophils. IL-5 induces eosinophil progenitor cell proliferation, terminal differentiation, and activation. In animal models of allergic diseases or helminth infection, IL-5 induces a massive proliferation of eosinophil progenitors in the bone marrow, promotes eosinophil recruitment with eotaxins, and prolongs eosinophil survival in local tissues. IL-5 regulates genes involved in the B cell terminal differentiation. IL-5 induces CD38-activated splenic B cells to differentiate into immunoglobulin M-secreting cells and go through m to g1 class switch recombination at the DNA level, resulting in immunoglobulin G1 (IgG1) production. IL-5 binds the IL-5R complex, which consists of an IL-5Rα chain specific for IL-5 and a common β-chain that is shared by the receptors for IL-3 and GM-CSF. The alpha subunit is required for ligand-specific binding whereas association with the beta subunit results in increased binding affinity. IL-5 plays important roles in the pathogenesis of asthma, hypereosinophilic syndromes, and eosinophil-dependent inflammatory diseases.
Product Details
- Source
- Human IL-5, amino acid Ile20-Ser134 (Accession # P05113) was expressed in E.coli.
- Molecular Mass
- The 116 amino acid recombinant protein has a predicted molecular mass of approximately 13.3 kD. The DTT-reduced protein migrates at approximately 14 kD and and the non-reduced protein migrates at approximately 28 kD by SDS-PAGE. The predicted N-terminal amino acid is Ile.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS pH 7.4, 500mM NaCl, 10% Glycerol
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.05 - 0.25 ng/mL as measured by the ability of the protein to induce proliferation of TF 1 human erythroleukemic cells.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Activated Th2 cells, mast cells, eosinophils, and basophils. In addition, newly identified IL-5 producing cells are: natural helper cells or nuocytes (lately identified as type II innate lymphoid cells), MPPtype2, and Ih2 cells.
- Function
- IL-5 regulates the production of eosinophils from purified hematopoieitic progenitors and regulates genes involved in the B cell terminal differentiation. IL-25 and IL-33 induce Th2 cytokines, among them IL-5.
- Interaction
- Eosinophils, B cells, basophils, and activated T cells
- Ligand/Receptor
- Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
- Bioactivity
- Measured by its ability to induce proliferation of TF-1 human erythroleukemic cells
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Lopez AF, et al. 1988. J Exp Med. 167:219-24.
- Horikawa K and Takatsu K. 2006. Immunology. 118:497.
- Moro K, et al. 2010. Nature. 463:540-4.
- Neill DR, et al. 2010. Nature. 464:1367-70.
- Saenz SA, et al. 2010. Nature. 464:1362-6.
- Ikutani M, et al. 2012. J Immunol. 188:703-13.
- Yasuda K, et al. 2012. Proc Natl Acad Sci U S A. 109:3451-6.
- Gene ID
- 3567 View all products for this Gene ID
- UniProt
- View information about IL-5 on UniProt.org