Pricing & Availability
Technical data sheet
- Regulatory Status
- RUO
- Other Names
- Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 715104 | 20 µg | $253.00 | |||
This product is not available for shipping outside of the United States.
Description
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.
Product Details
- Source
- Human IL-6, amino acids Pro29-Met212 (Accession# NM_000600), was expressed in E. coli.
- Molecular Mass
- The 184 amino acid recombinant protein has a predicted molecular mass of 20.9 kD. The predicted N-terminal amino acid is Pro.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg as determined by the dose-dependent stimulation of the proliferation of murine 7TD1 cells.
- Application
-
Bioassay
- Application Notes
-
This product is reactive with human and mouse.
- Product Citations
-
Antigen Details
- Structure
- Cytokine
- Distribution
- T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells.
- Function
- Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
- Interaction
- T cells, B cells, hepatocytes, cholinergic neurons.
- Ligand/Receptor
- Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130, gp130)
- Bioactivity
- Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Stem Cells, Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219. - Gene ID
- 3569 View all products for this Gene ID
- UniProt
- View information about IL-6 on UniProt.org