- Regulatory Status
- RUO
- Other Names
- Interleukin 7, Lymphopoietin-1 (LP-1)
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Proliferation of PHA-activated human PBL induced by human IL-7.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 581902 | 10 µg | $218.00 | |||
| 581904 | 25 µg | $382.00 | |||
| 581906 | 100 µg | $1014.00 | |||
| 581908 | 500 µg | $2636.00 | |||
IL-7 was initially described as a stromal derived factor which is capable of inducing the growth of pre-B cells in vitro. IL-7 acts on a variety of cells through its receptor (IL-7R), a heterodimer consisting of IL-7Rα (CD127) and a common γc chain (CD132) shared by other cytokine (IL-2, IL-4, IL-9, IL-15, and IL-21) receptors. In addition, IL-7Rα is shared with TSLP. The generation of IL-7-deficient and IL-7Rα-deficient mice and monoclonal antibody blocking experiments confirmed the requirement of IL-7 for B-cell development in mice. Nevertheless, mutations in the α chain of the IL-7 receptor in patients with severe combined immunodeficiency (SCID) confirmed that IL-7 is indispensable for T-cell development in humans. However, the presence of B cells in these individuals suggests important differences between the role of IL-7 in murine and human lymphocyte development. Thus, although human B-cell development does not appear to require IL-7, immature human B cells do proliferate in response to IL-7. Nevertheless, most recent information suggests that IL-7 dependence in human lymphopoiesis increases during the progression of ontogeny in cord blood and bone marrow. IL-7 can be associated to hepatocyte grow factor (HGFβ) to form a hybrid cytokine (IL-7/HGFβ), which induces greater proliferation of CFU-S, SLPs, and pre–pro-B cells than does native IL-7. The hybrid cytokine signals through both IL-7R (IL-7Rα plus γc) and c-Met. IL-7 has a thymic antiapoptotic effect and induces the expression of antiapoptotic proteins Bcl-2 and Mcl-1 and the inhibition of proapoptotic proteins Bax and Bad. In addition, IL-7 is a key regulator of glucose uptake in T lymphocytes. TGF-β has been shown to down-regulate IL-7 mRNA and protein secretion from human bone marrow stromal cells. In addition, TGF-β inhibits IL-7-induced proliferation of pre-B cells.
Product Details
- Source
- Human IL-7, amino acids Asp26-His177 (Accession# NP_000871.1) was expressed in 293E cells.
- Molecular Mass
- The 165 amino acid recombinant protein has a predicted molecular mass of approximately 18.8 kD. The DTT-reduced and non-reduced protein migrate at approximately 20- 30 kD by SDS-PAGE. The C-terminal contains an 8His-(TG8HGGQ)-tag.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 =0.2 — 1.2 ng/ml, corresponding to a specific activity of 0.83 - 5 x 106 units/mg, as determined by PHA-activated human PBL proliferation induced by human IL-7 in a dose dependent manner.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Distribution
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IL-7 is produced by epithelial cells in thymus, bone marrow, and intestine. Additional sites of IL-7 production include epithelial goblet cells, keratinocytes, fetal liver, adult liver, dendritic cells, skeletal muscle cells, fibroblastic reticular cells, and follicular dendritic cells.
- Function
- IL-7 induces proliferation of human immature B cells, and it is critical for T-cell development and peripheral T-cell homeostasis.
- Interaction
- IL-7Rα is expressed on immature B cells through the early pre-B stage. The IL-77Rα is widely expressed in T cells, CD4, CD8 naïve and memory cells, and dendritic cells.
- Ligand/Receptor
- IL-7Rα (CD127), common gamma chain (γc or CD132).
- Cell Type
- B cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Park LS, et al. 1990. J. Exp. Med. 171:1073.
2. Fry TJ and Mackall CL, 2002. Blood 99:3892.
3. Lai L, et al. 2006. Blood 107:1776.
4. Link A, et al. 2007. Nat. Immunol. 8:1255.
5. Wofford J, et al. 2008. Blood 111:2101.
6. Parrish YK, et al. 2009. J. Immunol. 182:4255.
7. Saini M, et al. 2009. Blood 113:5793.
8. Guimond M, et al. 2009. Nat. Immunol. 10:149. - Gene ID
- 3574 View all products for this Gene ID
- UniProt
- View information about IL-7 on UniProt.org