- Regulatory Status
- RUO
- Other Names
- IL7, IL-7, interleukin-7, Interleukin 7, Lymphopoietin-1 (LP-1), PBGF
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Human IL-7 induces proliferation of PHA-activated human peripheral blood lymphocytes (PBL) in a dose-dependent manner. The ED50 for this effect is 0.1 – 1.5 ng/mL. -
Stability Testing for Recombinant Human IL-7. Recombinant human IL-7 was aliquoted in PBS and pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to induce the proliferation of PHA-activated human PBL in a dose-dependent manner. The ED50 for this effect is 0.1 – 1.5 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 569704 | 25 µg | $425.00 | |||
| 569706 | 100 µg | $1080.00 | |||
IL-7 was initially described as a stromal derived factor capable of inducing in vitro growth of pre-B cells. IL-7 acts on a variety of cells through its receptor (IL-7R), a heterodimer consisting of IL-7Rα (CD127) and a common γc chain (CD132) shared by other cytokine (IL-2, IL-4, IL-9, IL-15, and IL-21) receptors. The generation of IL-7-deficient and IL-7Rα-deficient mice and monoclonal antibody blocking experiments confirmed the requirement of IL-7 for B-cell development in mice. Mutations in the α chain of the IL-7 receptor in patients with severe combined immunodeficiency (SCID) confirmed that IL-7 is indispensable for T-cell development in humans. However, the presence of B cells in these individuals suggests important differences between the role of IL-7 in murine and human lymphocyte development. Thus, although human B-cell development does not appear to require IL-7, immature human B cells do proliferate in response to IL-7. Recent information suggests that IL-7 dependence in human lymphopoiesis increases during the progression of ontogeny in cord blood and bone marrow. Relative to IL-7 alone, greater proliferation of CFUs, SLPs and pre-pro B cells is observed by hybrid cytokine IL-7/HGFβ, as association of IL-7 and hepatocyte growth factor (HGFβ). This hybrid cytokine signals through both IL-7R (IL-7Rα plus γc) and c-Met. IL-7 has a thymic antiapoptotic effect and induces the expression of antiapoptotic proteins Bcl-2 and Mcl-1 and the inhibition of proapoptotic proteins Bax and Bad. In addition, IL-7 is a key regulator of glucose uptake in T lymphocytes. TGF-β has been shown to down-regulate IL-7 mRNA and protein secretion from human bone marrow stromal cells. In addition, TGF-β inhibits IL-7-induced proliferation of pre-B cells.
Product Details
- Source
- Recombinant human IL-7, amino acid Asp26-His177 (Accession No. P13232) was expressed in E. Coli. The N-terminal includes 6His.
- Molecular Mass
- The 171 amino acid recombinant protein has a predicted molecular mass of approximately 19.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 15 - 27 kD by SDS-PAGE. The N-terminal contains a 6His tag.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS and pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.1 – 1.5 ng/mL, as determined by a dose-dependent stimulation in a proliferation assay with PHA-activated human peripheral blood lymphocytes (PBL). Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. Results may vary depending on human donors.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
IL-7 is produced by epithelial cells in thymus, bone marrow, and intestine. Additional sites of IL-7 production include epithelial goblet cells, keratinocytes, fetal liver, adult liver, dendritic cells, skeletal muscle cells, fibroblastic reticular cells, and follicular dendritic cells
- Function
- IL-7 induces proliferation of human immature B cells, critical for T-cell development and maintenance of peripheral T-cell homeostasis
- Interaction
- IL-7Rα is expressed on immature B cells through the early pre-B stage. The IL-7Rα is widely expressed in T cells, CD4, CD8 naÏve and memory cells, and dendritic cells
- Ligand/Receptor
- IL-7Rα (CD127), common gamma chain (γc or CD132)
- Bioactivity
- Measured by its ability to induce PHA-activated human PBL proliferation
- Cell Type
- B cells, Hematopoietic stem and progenitors
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Park LS, et al. 1990. J Exp Med. 171:1073-89.
- Fry TJ & Mackall CL. 2002. Blood. 99:3892-904.
- Lai L, et al. 2006. Blood. 107:1776-84.
- Link A, et al. 2007. Nat Immunol. 8:1255-65.
- Wofford JA, et al. 2008. Blood. 111:2101-11.
- Parrish YK, et al. 2009. J Immunol. 182:4255-66.
- Saini M, et al. 2009. Blood. 113:5793-800.
- Guimond M, et al. 2009. Nat Immunol. 10:149-57.
- Gene ID
- 3574 View all products for this Gene ID
- UniProt
- View information about IL-7 on UniProt.org