- Regulatory Status
- RUO
- Other Names
- IL-7RA, IL-7 Receptor Subunit Alpha, Interleukin 7 Receptor, IL-7R-Alpha, CD127 Antigen, CDW127, Interleukin 7 Receptor Alpha Chain
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Immobilized recombinant human IL-7Rα, at 2 µg/mL, binds recombinant human IL-7 (Cat. No. 581902) in a dose dependent manner. The ED50 for this effect is 4 - 20 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 771002 | 10 µg | $89.00 | |||
| 771004 | 25 µg | $188.00 | |||
| 771006 | 100 µg | $522.00 | |||
| 771008 | 500 µg | $1746.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-7 was initially described as a stromal derived factor which is capable of inducing the growth of pre-B cells in vitro. IL-7 acts on a variety of cells through its receptor (IL-7R), a heterodimer consisting of IL-7Rα (CD127) and a common γc chain (CD132) shared by other cytokine (IL-2, IL-4, IL-9, IL-15, and IL-21) receptors. In addition, IL-7Rα is shared with TSLP. The generation of IL-7-deficient and IL-7Rα-deficient mice and monoclonal antibody blocking experiments confirmed the requirement of IL-7 for B-cell development in mice. Nevertheless, mutations in the α chain of the IL-7 receptor in patients with severe combined immunodeficiency (SCID) confirmed that IL-7 is indispensable for T-cell development in humans. However, the presence of B cells in these individuals suggests important differences between the role of IL-7 in murine and human lymphocyte development. Thus, although human B-cell development does not appear to require IL-7, immature human B cells do proliferate in response to IL-7. Nevertheless, most recent information suggests that IL-7 dependence in human lymphopoiesis increases during the progression of ontogeny in cord blood and bone marrow. IL-7 can be associated to hepatocyte grow factor (HGFβ) to form a hybrid cytokine (IL-7/HGFβ), which induces greater proliferation of CFU-S, SLPs, and pre–pro-B cells than does native IL-7. The hybrid cytokine signals through both IL-7R (IL-7Rα plus γc) and c-Met. Soluble forms of the IL-7R α chain (sCD127) have been identified. They are derived from alternative splicing and by release of membrane-bound sCD127. sCD127 acts as an antagonist and is increased by immune activation. sCD127 concentration is increased in Type I diabetes patients, and it is glycated. The glycated form does not act as an antagonist of IL-7–mediated T-cell expansion. Alterations of the soluble form of IL-7Ra influences risk of multiple sclerosis.
Product Details
- Molecular Mass
- The 463 amino acid recombinant protein has a predicted molecular mass of approximately 52.7 kD. The DTT-reduced and non-reduced protein migrate at approximately 70 kD and 140 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Glu.
- Purity
- > 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg (0.01 ng/µg) cytokine as determined by the LAL method.
- Concentration
- 10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes and larger are bottled at the concentration indicated on the vial.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized recombinant human IL-7Rα, at 2 µg/mL, binds recombinant human IL-7 (Cat. No. 581902) in a dose dependent manner. The ED50 for this effect is 4 - 20 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
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High levels of soluble IL-7Ra in plasma indicate pathologic T-cell immunity in autoimmune, inflammatory, and chronic viral diseases.
- Function
- Modulation of T-cell functions, development and homeostasis of T cells, promotes antigen-specific T-cell responses, generation of memory T cells.
- Ligand/Receptor
- IL-7, IL-7/HGFβ
- Bioactivity
- IL-7R inhibits the proliferation of TF-1 cells.
- Biology Area
- Stem Cells
- Molecular Family
- Soluble Receptors
- Antigen References
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- Park LS, et al. 1990. J. Exp. Med. 171:1073.
- Goodwin RG, et al. 1990. Cell. 60:941.
- Fry TJ and Mackall CL. 2002. Blood. 99:3892.
- Lai L, et al. 2006. Blood. 107:1776.
- Link A, et al. 2007. Nat. Immunol. 8:1255.
- Lundmark F, et al. 2007. Nat. Genet. 39:1108.
- Wofford J, et al. 2008. Blood. 111:2101.
- Parrish YK, et al. 2009. J. Immunol. 182:4255.
- Saini M, et al. 2009. Blood. 113:5793.
- Guimond M, et al. 2009. Nat. Immunol. 10:149.
- Monti P, et al. 2013. Diabetes. 62:2500.
- Lundtoft C, et al. 2017. PLoS Pathog. 13(6):e1006425.
- Gene ID
- 3575 View all products for this Gene ID
- UniProt
- View information about CD127 on UniProt.org