- Regulatory Status
- RUO
- Other Names
- Small inducible cytokine subfamily B, member 8 (SCYB8), Monocyte derived neutrophils chemotactic factor (MDNCF), Neutrophil-activating peptide 1 (NAP1), Granulocyte chemotactic protein 1 (GCP1), Chemokine, CXC motif, ligand 8, CXCL8
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 715404 | 25 µg | $253.00 | |||
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IL-8 (CXCL8) was originally recognized as a neutrophil-activating protein on the basis of two in vitro effects, chemotaxis and the release of granule enzymes. IL-8 is generated as a precursor of 99 amino acids. Once the mature cytokine is secreted, the amino-terminal is processed to yield several biologically active variants. The predominant variant consists of 72 amino acids. IL-8 is one of the ELR (Glu-Leu-Arg) motif-positive CXC-chemokines and shares similarities with angiogenic cytokines.
Product Details
- Source
- Human IL-8, amino acids Ser28-Ser99 (Accession# NM_000584) was expressed in E. coli.
- Molecular Mass
- The 72 amino acid recombinant protein has a predicted molecular mass of 8.4 kD. The predicted N-terminal amino acid is Ser.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Formulation
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Activity
- Bioactivity was measured by its property to chemoattract human peripheral blood neutrophils in a dose dependent manner. The chemotactic activity was detected from 10 to 100 ng/ml.
- Application
-
Bioassay
- Product Citations
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Antigen Details
- Structure
- Chemokine
- Distribution
- IL-8 is expressed by neutrophils, mococytes, macrophages, mast cells, endothelial cells, fibroblasts, keratinocytes, synovial cells, chondrocytes, epithelial cells, megakariocytes, melanocytes, hepatocytes, mesangial, and tumor cells. Most primary and metastatic solid tumors, such as breast, uterine, prostate, colon, and pancreatic carcinomas, melanoma, and glioblastoma, constitutively express IL-8.
- Function
- CXCL8 has diverse functions in immune surveillance, inflammation, and angiogenesis. CXCL8 directly modulates endothelial cell proliferation and migration, thus promoting angiogenesis. IL-8 modulates myelopoiesis by suppressing the myeloid progenitor proliferation. IL-8 also modulates the homing of hematopoitic progenitors by affecting the secretion of MMPs and TIMPs from bone marrow and peripheral blood CD34+ cells. Monocytes and macrophages generate IL-8 upon stimulation with IL-1α, IL-1β, TNF-α, IL-3, GMCSF, endotoxin, lectins, phorbol esters, immune complexes, and phagocytosis. Opsonized particles are also a prime stimulus for IL-8 production by neutrophils.
- Interaction
- Neutrophils, basophils, highly cytotoxic CD8 T cells, megakariocytes, endothelial cells.
- Ligand/Receptor
- CXCR1 (CDw128a) and CXCR2.
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Hess C, et al. 2004. Blood 104:3463.
2. Emadi S, et al. 2005. Blood 105:464.
3. Matsuo Y, et al. 2009. Int. J. Cancer 4:853.
4. Drewniak A, et al. 2010. Blood 115:4588. - Gene ID
- 3576 View all products for this Gene ID
- UniProt
- View information about IL-8 on UniProt.org